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anti-Human IL17A antibody for ELISpot (Capture)

Recommended IL17A Antibody (supplied by: Log in to see )

Antigen
Interleukin 17A (IL17A) Antibodies
  • ChIL-17
  • Ctla-8
  • CTLA-8
  • CTLA8
  • Ctla8
  • IL-17
  • IL-17A
  • IL-17F
  • IL17
  • Il17
  • IL17A
Reactivity
Human
430
194
72
10
5
5
1
Host
Mouse
247
207
111
44
8
3
1
Clonality
Monoclonal
Conjugate
This IL17A antibody is un-conjugated
71
34
34
24
17
16
14
11
8
7
7
7
7
7
7
7
7
7
6
6
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
Application
ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture)), Neutralization (Neut)
345
312
193
59
43
41
24
22
19
15
11
10
9
9
9
7
7
6
4
4
4
3
2
2
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN282544
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN281663 ELISA (Capture) ELISPOT (Capture) FACS IHC (fro) IP ICS Mouse IgG1 kappa Log in to see 64CAP17 1
0.0008709465 ABIN1722445 ELISA (Capture) ELISPOT (Capture) Mouse IgG2b Log in to see B-C49

Top referenced anti-IL17A antibody for ELISpot (Capture)

Similar anti-IL17A Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 3 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 2 Antibodies
Confocal Microscopy (CM) 2 Antibodies
Depletion (Dep) 1 Antibodies
ELISA 193 Antibodies
ELISA (Capture) 7 Antibodies
ELISA (Detection) 8 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 118 Antibodies
Functional Studies (Func) 9 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies

General

Antigen Interleukin 17A (IL17A) Antibodies
Reactivity Human
(430), (194), (72), (10), (5), (5), (1)
Host Mouse
(247), (207), (111), (44), (8), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL17A antibody is un-conjugated
(71), (34), (34), (24), (17), (16), (14), (11), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture)), Neutralization (Neut)
(345), (312), (193), (59), (43), (41), (24), (22), (19), (15), (11), (10), (9), (9), (9), (7), (7), (6), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL17A Antibody

Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN282544) Images
Specificity The antibody reacts with human IL-17A, the antibody has been reported to cross react with Rhesus monkey IL-17A, as verified by intracellular staining experiments. The 64CAP17 antibody is a neutralizing antibody. This antibody has been shown to have no reactivity to human IL-17F. Reactivity to other members of the IL-17 family has not been evaluated. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells, the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Additionally, activated human CD4+ T cells have been found to produce the IL-17A/F heterodimer, as well as the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion, IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). 64CAP17 can be used to detect IL-17 heterodimers by immunoprecipitation followed by immunoblot withH17F10A7 anti-IL17F monoclonal antibody.

The 64CAP17 has been shown to react to rhesus and marmoset primates.
Characteristics The eBio64CAP17 antibody reacts with human IL-17A, the antibody has been reported to cross react with Rhesus monkey IL-17A, as verified by intracellular staining experiments. The eBio64CAP17 antibody is a neutralizing antibody. This antibody has been shown to have no reactivity to human IL-17F. Reactivity to other members of the IL-17 family has not been evaluated. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells, the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T- lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL- 17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor. IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. Additionally, activated human CD4+ T cells have been found to produce the IL-17A/F heterodimer, as well as the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion, IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). eBio64CAP17 can be used to detect IL-17 heterodimers by immunoprecipitation followed by immunoblot withH17F10A7 anti-IL17F monoclonal antibody. The eBio64CAP17 has been shown to react to rhesus and marmoset primates.
Synonyms: Synonyms: Interleukin-17A, IL17A
Purification Affinity Purified
Endotoxin Level <0.001 ng endotoxin/μg
Grade Functional Grade
Clone 64CAP17
Isotype IgG1 kappa

Target Details IL17A

Product Details anti-IL17A Antibody Application Details Handling References for anti-IL17A Antibody (ABIN282544) Images back to top
Antigen
Alternative Name IL-17A (IL17A Antibody Abstract)
Pathways TCR Signaling

Application Details

Product Details anti-IL17A Antibody Target Details IL17A Handling References for anti-IL17A Antibody (ABIN282544) Images back to top
Application Notes Applications reported: The eBio64CAP17 antibody has been reported for use as the capture antibody in a human IL-17A ELISA and ELISPOT assay, for neutralization of IL-17A bioactivity, and for intracellular staining of IL-17A-producing cells.

Applications tested: The Affinity Purified eBio64CAP17 antibody has been tested as the capture antibody in a sandwich ELISA for measurement of human IL-17A protein levels, in combination with the biotinylated eBio64DEC17 antibody (13-7179) for detection and recombinant human IL-17A (14-8179) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5 - 2.0 μg μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The Functional Grade Purified eBio64CAP17 antibody has been tested by LAL assay to verify lowest endotoxin levels and has been tested for neutralization of IL-17Abioactivity and for ELISPOT capture. For in vitro neutralization, the eBio64CAP17 antibody at less than or equal to 63 ng/mL has been found to neutralize by 50 % the biological effect of 5 ng/mL human IL-17A, based on induction of IL-6 secretion by the NHDF cell line. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Restrictions For Research Use only

Handling

Product Details anti-IL17A Antibody Target Details IL17A Application Details References for anti-IL17A Antibody (ABIN282544) Images back to top
Concentration 1 mg/mL
Buffer aqueous buffer, no sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C

References for anti-IL17A Antibody (ABIN282544)

Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling Images back to top
Product cited in:

Kap, van Meurs, van Driel, Koopman, Melief, Brok, Laman, t Hart: "A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 12, pp. 1159-67, 2009

Images

Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN282544) back to top
Supplier Images
 image for anti-IL17A antibody (Interleukin 17A) (ABIN282544) Human IL-17A ELISPOT Assay. Left: Human PBMCs cultured for 24 hrs (no mitogen). Right...
 image for anti-IL17A antibody (Interleukin 17A) (ABIN282544) anti-Interleukin 17A (IL17A) antibody (Image 2)