We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)||Cow (Bovine)|
|Western Blotting (WB)||83 Antibodies||18 Antibodies||11 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies||3 Antibodies||6 Antibodies||6 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||31 Antibodies||2 Antibodies||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies||11 Antibodies|
|Immunofluorescence (IF)||10 Antibodies||1 Antibodies|
|Antigen||Axin 1 (AXIN1) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AXIN1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|1 reference available|
|Supplier||Log in to see|
Product Details anti-AXIN1 AntibodyTarget Details AXIN1 Application Details Handling References for anti-AXIN1 Antibody (ABIN374189) Images
|Specificity||This antibody detects Axin-1 at C-term.|
|Purification||Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide|
|Immunogen||Peptide from C Terminus of the protein sequence according to NP_003493.1, NP_851393.1. Genename: AXIN1|
Target Details AXIN1Product Details anti-AXIN1 Antibody Application Details Handling References for anti-AXIN1 Antibody (ABIN374189) Images back to top
|Alternative Name||Axin-1 (AXIN1 Antibody Abstract)|
|Background||Axin 1 is a negative regulator of Wnt signaling and is also associated with cancer. Axin 1 contains RGS (Regulator of G protein signaling domain) and Axin_b-cat_bind (Axin beta-catenin binding domain).Synonyms: AXIN, AXIN1, Axis inhibition protein 1|
Application DetailsProduct Details anti-AXIN1 Antibody Target Details AXIN1 Handling References for anti-AXIN1 Antibody (ABIN374189) Images back to top
Peptide ELISA: Limit dilution 1: 128000. Western blot: Preliminary experiments gave bands at approx 150 kDa and 22-20 kDa inHuman Brain (Hippocampus) lysates after 0.1 μg/mL antibody staining. Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Adrenal Gland showsmembranous and fibrous staniing in and around secretory cells of the zona fasciculata inthe cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AXIN1 Antibody Target Details AXIN1 Application Details References for anti-AXIN1 Antibody (ABIN374189) Images back to top
|Buffer||Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.|
References for anti-AXIN1 Antibody (ABIN374189)Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling Images back to top
Zeng, Fagotto, Zhang et al.: "The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation." in: Cell, Vol. 90, Issue 1, pp. 181-92, 1997 (PubMed).
ImagesProduct Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling References for anti-AXIN1 Antibody (ABIN374189) back to top