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anti-Human AXIN1 antibody for Western Blotting

Recommended AXIN1 Antibody (supplied by: Log in to see )

Antigen
Axin 1 (AXIN1) Antibodies
  • AI316800
  • Axin
  • AXIN
  • axin
  • AXIN1
  • AXIN1-RGS
  • Fu
  • fused
  • Kb
  • Ki
  • kinky
  • knobbly
  • LOC100226467
  • PPP1R49
  • wu:fi22e03
  • wu:fj14c08
  • wu:fk11b03
  • xAxin
  • zgc:109872
Epitope
C-Term, AA 717-746
47
15
15
15
13
10
4
4
3
3
2
2
2
2
2
1
1
Reactivity
Human
111
35
27
5
4
2
1
1
1
1
1
Host
Rabbit
85
19
14
Clonality
Polyclonal
Conjugate
This AXIN1 antibody is un-conjugated
8
8
6
5
5
5
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
86
64
31
15
11
10
5
3
3
1
Supplier
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Catalog No. ABIN950582
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN783749 EIA WB Rabbit C-Term Log in to see Polyclonal 2
0.12303223 ABIN567615 EIA IF IP WB Mouse IgG2b Log in to see AT1A4 4
0.12303223 ABIN2775612 WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN2854479 WB Rabbit IgG C-Term Log in to see Polyclonal
0.12303223 ABIN1538316 WB Rabbit Ig AA 330-359, Center Log in to see Polyclonal
0.12303223 ABIN2705551 WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN740295 IF (p) IHC (p) WB Rabbit IgG AA 830-862 Log in to see Polyclonal
0.12303223 ABIN2159397 WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.12303223 ABIN934064 IF ELISA WB Mouse IgG2b kappa AA 546-752 Log in to see AT1A4
0.12303223 ABIN2159398 IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.12303223 ABIN2157050 WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2156029 ELISA WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN1030273 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011806369 ABIN655972 IHC (p) WB Rabbit Ig AA 710-738, C-Term Log in to see Polyclonal 3
0.0011806369 ABIN393560 ELISA WB Mouse IgG2a kappa AA 643-741 Log in to see 2B11 5
0.0011806369 ABIN184720 IHC ELISA WB Goat C-Term Log in to see Polyclonal 1
0.0011806369 ABIN959619 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0011806369 ABIN1974454 IHC ELISA WB PE Rabbit IgG C-Term, AA 717-746 Log in to see Polyclonal
0.0011806369 ABIN1974455 ELISA WB PE Rabbit IgG Internal Region, AA 329-359 Log in to see Polyclonal
0.0011806369 ABIN200294 WB Rabbit Internal Region Log in to see Polyclonal

Top referenced anti-AXIN1 antibody for Western Blotting

Similar anti-AXIN1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 1 Antibodies
ELISA 63 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 83 Antibodies

General

Antigen Axin 1 (AXIN1) Antibodies
Epitope C-Term, AA 717-746
(47), (15), (15), (15), (13), (10), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Reactivity Human
(111), (35), (27), (5), (4), (2), (1), (1), (1), (1), (1)
Host Rabbit
(85), (19), (14)
Clonality
Polyclonal
Conjugate This AXIN1 antibody is un-conjugated
(8), (8), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(86), (64), (31), (15), (11), (10), (5), (3), (3), (1)
Pubmed 2 references available
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Product Details anti-AXIN1 Antibody

Target Details AXIN1 Application Details Handling References for anti-AXIN1 Antibody (ABIN950582) Images
Specificity This antibody reacts to Axin-1.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography on Protein A
Immunogen KLH conjugated synthetic peptide between 717-746 amino acids from the C-terminal region of human AXIN1
Isotype Ig

Target Details AXIN1

Product Details anti-AXIN1 Antibody Application Details Handling References for anti-AXIN1 Antibody (ABIN950582) Images back to top
Antigen
Alternative Name Axin-1 (AXIN1 Antibody Abstract)
Background This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene.Synonyms: AXIN, AXIN1, Axis inhibition protein 1
Gene ID 8312
NCBI Accession NP_003493
Pathways WNT Signaling

Application Details

Product Details anti-AXIN1 Antibody Target Details AXIN1 Handling References for anti-AXIN1 Antibody (ABIN950582) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details References for anti-AXIN1 Antibody (ABIN950582) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-AXIN1 Antibody (ABIN950582)

Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling Images back to top
Background publications

Sue Ng, Mahmoudi, Li, Hatzis, Boersema, Mohammed, Heck, Clevers: "MAP3K1 functionally interacts with Axin1 in the canonical Wnt signalling pathway." in: Biological chemistry, Vol. 391, Issue 2-3, pp. 171-80, 2010

Li, Lin, Wang, Lian, Lu, Guo, Ruan, Wang, Ye, Han, Lin: "Axin determines cell fate by controlling the p53 activation threshold after DNA damage." in: Nature cell biology, Vol. 11, Issue 9, pp. 1128-34, 2009

Images

Product Details anti-AXIN1 Antibody Target Details AXIN1 Application Details Handling References for anti-AXIN1 Antibody (ABIN950582) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AXIN1 antibody (Axin 1) (C-Term) (ABIN950582) AXIN1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin ...
Western Blotting (WB) image for anti-AXIN1 antibody (Axin 1) (C-Term) (ABIN950582) AXIN1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35µg/la...