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anti-Human CAMK2A antibody for Immunoprecipitation

Recommended CAMK2A Antibody (supplied by: Log in to see )

Antigen
Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
  • camk2
  • camk2b
  • CAMKA
  • CaMKII
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • R74975
  • zgc:112538
  • zgc:123320
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
123
80
66
9
9
8
5
5
4
3
3
2
2
2
1
1
Host
Mouse
115
53
18
Clonality
Monoclonal
Conjugate
This CAMK2A antibody is un-conjugated
6
6
6
6
6
5
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
175
86
55
42
29
25
21
8
6
2
2
2
2
1
Supplier
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Catalog No. ABIN492419
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008941957 ABIN453934 EIA IHC (fro) IF IP RIA WB Mouse IgG1 Log in to see 6G9
0.0008941957 ABIN453935 EIA IHC (fro) IF IP WB Mouse IgG1 pThr286 Log in to see 22B1
0.0008941957 ABIN492420 EIA IHC (fro) IF IP WB Mouse IgG1 pThr286 Log in to see 22B1
0.0008941957 ABIN451525 ICC IF IHC (p) IP IHC ELISA WB Mouse IgG1 kappa pThr286, pThr287 Log in to see 22B1
0.0008941957 ABIN151484 ICC IF IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see 6G9

Similar anti-CAMK2A Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
Dot Blot (DB) 2 Antibodies 2 Antibodies
ELISA 35 Antibodies 27 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 3 Antibodies
Immunofluorescence (IF) 34 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Radioimmunoassay (RIA) 23 Antibodies
Western Blotting (WB) 81 Antibodies

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(123), (80), (66), (9), (9), (8), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1)
Host Mouse
(115), (53), (18)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CAMK2A antibody is un-conjugated
(6), (6), (6), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
(175), (86), (55), (42), (29), (25), (21), (8), (6), (2), (2), (2), (2), (1)
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Product Details anti-CAMK2A Antibody

Target Details CAMK2A Application Details Handling Images
Cross-Reactivity (Details) Species reactivity (tested):Mouse, Rat, Bovine, Human.
Characteristics Synonyms: CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-IIsubunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain
Purification Protein G Chromatography
Immunogen Partially purified Rat CaMKII
Clone 6G9
Isotype IgG1

Target Details CAMK2A

Product Details anti-CAMK2A Antibody Application Details Handling Images back to top
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca 2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968
Gene ID 25400
UniProt P11275
Research Area Kinases/Phosphatases
Pathways WNT Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-CAMK2A Antibody Target Details CAMK2A Handling Images back to top
Application Notes RIA. ELISA. Immunflourescence. Immunoprecipitation. Immunohistochemistry. Western blot: 0.1 μg/mL of this CamKIIA antibody was needed for detection of CaMKII in 20 μg of Rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-MouseIgG: AP as the secondary antibody.
Restrictions For Research Use only

Handling

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS pH 7.4 containing 0.09 % Sodium Azide in 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha) (ABIN492419) anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibody
Immunofluorescence (IF) image for anti-CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha) (ABIN492419) anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibody (Image 2)