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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)|
|Western Blotting (WB)||118 Antibodies||65 Antibodies||81 Antibodies||9 Antibodies|
|Radioimmunoassay (RIA)||4 Antibodies||23 Antibodies||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies||27 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||12 Antibodies||15 Antibodies||28 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||13 Antibodies||29 Antibodies|
|Antigen||Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CAMK2A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-CAMK2A AntibodyTarget Details CAMK2A Application Details Handling References for anti-CAMK2A Antibody (ABIN452427) Images
|Specificity||This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).|
Species reactivity (expected):Rat, Canine.
Species reactivity (tested):Human, Mouse.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide with sequence from the internal region of the protein sequence|
Target Details CAMK2AProduct Details anti-CAMK2A Antibody Application Details Handling References for anti-CAMK2A Antibody (ABIN452427) Images back to top
|Alternative Name||CAMK2A (CAMK2A Antibody Abstract)|
|Background||Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning. CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1 % of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30 % of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968|
|Pathways||WNT Signaling, Interferon-gamma Pathway|
Application DetailsProduct Details anti-CAMK2A Antibody Target Details CAMK2A Handling References for anti-CAMK2A Antibody (ABIN452427) Images back to top
Peptide ELISA: 1/64000. Western blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CAMK2A Antibody Target Details CAMK2A Application Details References for anti-CAMK2A Antibody (ABIN452427) Images back to top
|Buffer||Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-CAMK2A Antibody (ABIN452427)Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling Images back to top
Lee, Lin, Luo et al.: "Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and ..." in: Molecular pharmacology, Vol. 73, Issue 5, pp. 1454-64, 2008 (PubMed).
ImagesProduct Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling References for anti-CAMK2A Antibody (ABIN452427) back to top