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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Chicken||Xenopus laevis|
|Immunofluorescence (fixed cells) (IF/ICC)||6 Antibodies||2 Antibodies||2 Antibodies|
|Simple Western (SimWes)||1 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||391 Antibodies||188 Antibodies||164 Antibodies||33 Antibodies||20 Antibodies|
|RNA Interference (RNAi)||2 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Luminex Assay (LMNX)||8 Antibodies|
|Immunoprecipitation (IP)||66 Antibodies||52 Antibodies|
|Antigen||Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies|
|Reactivity||Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis Alternatives|
|Conjugate||This CTNNB1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-CTNNB1 AntibodyTarget Details CTNNB1 Application Details Handling References for anti-CTNNB1 Antibody (ABIN181394) Images
This antibody detects Catenin-beta.
Cellular Localization: Cytoplasmic and cell membrane
|Purification||Protein A chromatography|
|Immunogen||A synthetic peptide from the C-terminus of the beta-catenin protein.|
Target Details CTNNB1Product Details anti-CTNNB1 Antibody Application Details Handling References for anti-CTNNB1 Antibody (ABIN181394) Images back to top
|Alternative Name||Catenin beta-1 (CTNNB1 Antibody Abstract)|
|Background||The catenins (α, β and γ) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. β- catenin also binds to N-cadherin and coimmunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between α-actinin and α-catenin. Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.Synonyms: Beta-catenin, CTNNB, CTNNB1|
|Pathways||WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling References for anti-CTNNB1 Antibody (ABIN181394) Images back to top
Immunoprecipitation (Native verified): Use Protein A, Ab 10 g/mg protein lysate. Western Blotting: 5-10 μg/mL for 2 hrs at RT. Immunohistology (Formalin/paraffin): Use Ab at 1-2 μg/mL for 30 min at RT. [Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0), for10-20 min followed by cooling at RT for 20 min. ]Positive Control: MCF-7 cells. Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details References for anti-CTNNB1 Antibody (ABIN181394) Images back to top
|Buffer||10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer.|
References for anti-CTNNB1 Antibody (ABIN181394)Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling Images back to top
Koslov, Maupin, Pradhan, Morrow, Rimm: "Alpha-catenin can form asymmetric homodimeric complexes and/or heterodimeric complexes with beta-catenin." in: The Journal of biological chemistry, Vol. 272, Issue 43, pp. 27301-6, 1997
ImagesProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling References for anti-CTNNB1 Antibody (ABIN181394) back to top