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|Application / Reactivity||Human|
|Chromatin Immunoprecipitation (ChIP)||3 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||13 Antibodies|
|Flow Cytometry (FACS)||65 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||32 Antibodies|
|Immunofluorescence (IF)||100 Antibodies|
|Immunohistochemistry (IHC)||117 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||81 Antibodies|
|Immunoprecipitation (IP)||62 Antibodies|
|Luminex Assay (LMNX)||8 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies|
|Antigen||Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies|
|Conjugate||This CTNNB1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|3 references available|
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Product Details anti-CTNNB1 AntibodyTarget Details CTNNB1 Application Details Handling References for anti-CTNNB1 Antibody (ABIN969072) Images
|Immunogen||Purified recombinant fragment of human CTNNB1 expressed in E. coli.|
Target Details CTNNB1Product Details anti-CTNNB1 Antibody Application Details Handling References for anti-CTNNB1 Antibody (ABIN969072) Images back to top
|Alternative Name||CTNNB1 (CTNNB1 Antibody Abstract)|
|Background||The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues and bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development.|
|Molecular Weight||85 kDa|
Application DetailsProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling References for anti-CTNNB1 Antibody (ABIN969072) Images back to top
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details References for anti-CTNNB1 Antibody (ABIN969072) Images back to top
|Buffer||Ascitic fluid containing 0.03 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 4 °C or at -20 °C for long term.|
References for anti-CTNNB1 Antibody (ABIN969072)Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling Images back to top
|Product cited in:||
Bougatef, Ouerhani, Moussa et al.: "Prevalence of mutations in APC, CTNNB1, and BRAF in Tunisian patients with sporadic colorectal cancer." in: Cancer genetics and cytogenetics, Vol. 187, Issue 1, pp. 12-8, 2008 (PubMed).
Guo, Liu, Yang et al.: "Relation among p130Cas, E-cadherin and beta-catenin expression, clinicopathologic significance and prognosis in human hepatocellular carcinoma." in: Hepatobiliary & pancreatic diseases international : HBPD INT, Vol. 7, Issue 5, pp. 490-6, 2008 (PubMed).
Rubinfeld, Souza, Albert et al.: "The APC protein and E-cadherin form similar but independent complexes with alpha-catenin, beta-catenin, and plakoglobin." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5549-55, 1995 (PubMed).
ImagesProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling References for anti-CTNNB1 Antibody (ABIN969072) back to top