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anti-Human CTNNB1 antibody for Immunocytochemistry

Recommended CTNNB1 Antibody (supplied by: Log in to see )

Antigen
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies
  • armadillo
  • beta-catenin
  • Bfc
  • Catnb
  • CHBCAT
  • CTNNB
  • ctnnb
  • CTNNB1
  • ctnnb1
  • id:ibd2058
  • Jup
  • LOC100217813
  • Mesc
  • MRD19
  • wu:fb73e10
  • wu:fi81c06
  • wu:fk25h01
Reactivity
Human
445
109
96
28
21
13
13
6
5
4
4
2
1
1
1
Host
Mouse
316
153
2
2
Clonality
Monoclonal
Conjugate
This CTNNB1 antibody is un-conjugated
15
12
9
6
6
6
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
402
152
115
100
84
66
65
31
16
13
8
4
3
3
2
1
1
1
Supplier
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Catalog No. ABIN969072
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.69106555 ABIN457419 ICC FACS IP WB Mouse IgG1 C-Term, AA 441-448 Log in to see EM-22 3
0.69106555 ABIN1842941 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010161072 ABIN189298 ICC IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see E247
0.0010161072 ABIN3044345 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 1-19, N-Term Log in to see Polyclonal
0.0010161072 ABIN211079 ICC IP WB Rabbit IgG AA 29-49 Log in to see Polyclonal
0.0010161072 ABIN574434 ICC FACS IHC (p) IP WB Mouse IgG1 Log in to see EM-22
0.0010161072 ABIN215673 ICC IP ELISA WB Mouse IgG2b Log in to see
0.0010161072 ABIN364501 ICC IHC (p) IP WB Mouse IgG AA 758-771 Log in to see
0.0010161072 ABIN1494121 ICC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0010161072 ABIN200357 ICC IP IHC WB Mouse IgG AA 758-771 Log in to see
0.0010161072 ABIN2619644 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 1-19 Log in to see Polyclonal
0.0010161072 ABIN604612 ICC ELISA WB Mouse IgG1 Log in to see
0.0010161072 ABIN600588 ICC IHC ELISA WB Mouse IgG1, kappa AA 36-44 Log in to see
0.0010161072 ABIN600589 ICC IHC ELISA WB Mouse IgG1, kappa AA 36-44 Log in to see
0.0010161072 ABIN1975812 ICC IHC ELISA WB PE Rabbit IgG C-Term, AA 653-681 Log in to see Polyclonal
0.0010161072 ABIN215671 ICC IHC (p) ELISA WB Mouse IgG1 AA 35-50 Log in to see
0.0010161072 ABIN1964799 ICC IHC ELISA WB APC Rabbit IgG C-Term, AA 653-681 Log in to see Polyclonal
0.0010161072 ABIN1962422 ICC IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 653-681 Log in to see Polyclonal
0.0010161072 ABIN3020927 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN1858529 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 439-675 Log in to see Polyclonal

Top referenced anti-CTNNB1 antibody for Immunocytochemistry

Similar anti-CTNNB1 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 3 Antibodies
ELISA 145 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 65 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 98 Antibodies
Immunohistochemistry (IHC) 116 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 80 Antibodies
Immunoprecipitation (IP) 62 Antibodies
Luminex Assay (LMNX) 8 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies

General

Antigen Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies
Reactivity Human
(445), (109), (96), (28), (21), (13), (13), (6), (5), (4), (4), (2), (1), (1), (1)
Host Mouse
(316), (153), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CTNNB1 antibody is un-conjugated
(15), (12), (9), (6), (6), (6), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(402), (152), (115), (100), (84), (66), (65), (31), (16), (13), (8), (4), (3), (3), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CTNNB1 Antibody

Target Details CTNNB1 Application Details Handling References for anti-CTNNB1 Antibody (ABIN969072) Images
Purification Ascites
Immunogen Purified recombinant fragment of human CTNNB1 expressed in E. coli.
Clone 4D5
Isotype IgG1

Target Details CTNNB1

Product Details anti-CTNNB1 Antibody Application Details Handling References for anti-CTNNB1 Antibody (ABIN969072) Images back to top
Antigen
Alternative Name CTNNB1 (CTNNB1 Antibody Abstract)
Background The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues and bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development.
Molecular Weight 85 kDa
Gene ID 1499
HGNC 1499
Pathways WNT Signaling

Application Details

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling References for anti-CTNNB1 Antibody (ABIN969072) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details References for anti-CTNNB1 Antibody (ABIN969072) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CTNNB1 Antibody (ABIN969072)

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling Images back to top
Product cited in:

Bougatef, Ouerhani, Moussa, Kourda, Coulet, Colas, Lahely, Najjar, Ben Jilani, Benammar-Elgaaied, Soubrier, Marrakchi: "Prevalence of mutations in APC, CTNNB1, and BRAF in Tunisian patients with sporadic colorectal cancer." in: Cancer genetics and cytogenetics, Vol. 187, Issue 1, pp. 12-8, 2008

Guo, Liu, Yang, Zhang, Yao: "Relation among p130Cas, E-cadherin and beta-catenin expression, clinicopathologic significance and prognosis in human hepatocellular carcinoma." in: Hepatobiliary & pancreatic diseases international : HBPD INT, Vol. 7, Issue 5, pp. 490-6, 2008

Background publications

Rubinfeld, Souza, Albert, Munemitsu, Polakis: "The APC protein and E-cadherin form similar but independent complexes with alpha-catenin, beta-catenin, and plakoglobin." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5549-55, 1995

Images

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling References for anti-CTNNB1 Antibody (ABIN969072) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CTNNB1 antibody (Catenin (Cadherin-Associated Protein), beta 1, 88kDa) (ABIN969072) Figure 1: Flow cytometric analysis of A549 cells using CTNNB1 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-CTNNB1 antibody (Catenin (Cadherin-Associated Protein), beta 1, 88kDa) (ABIN969072) Figure 2: Immunofluorescence analysis of A549 (left) and SK-BR-3 (right) cells using ...
Immunohistochemistry (IHC) image for anti-CTNNB1 antibody (Catenin (Cadherin-Associated Protein), beta 1, 88kDa) (ABIN969072) Figure 3: Immunohistochemical analysis of paraffin-embedded human Placenta tissues us...
Western Blotting (WB) image for anti-CTNNB1 antibody (Catenin (Cadherin-Associated Protein), beta 1, 88kDa) (ABIN969072) Figure 4: Western blot analysis using CTNNB1 mouse mAb against CTNNB1-hIgGFc transfec...