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anti-Human FZD6 antibody for Immunocytochemistry

Recommended FZD6 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 6 (FZD6) Antibodies
  • Frizzled-6
  • frizzled-6
  • frizzled6
  • frz6
  • FZ-6
  • Fz6
  • fz6
  • FZ6
  • HFZ6
  • NDNC10
  • Xfrz6
  • zgc:65879
  • zgc:77440
Epitope
N-Term, Extracellular Domain
18
15
13
10
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
62
16
7
5
1
Host
Rabbit
61
4
4
1
Clonality
Polyclonal
Conjugate
This FZD6 antibody is un-conjugated
4
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
42
35
14
13
9
5
4
2
1
1
1
1
1
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Catalog No. ABIN1048614
€ 429.52
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010115753 ABIN1734470 ICC IHC (p) Rabbit IgG N-Term, Extracellular Domain Log in to see Polyclonal
0.0010115753 ABIN2886365 ICC Rabbit AA 167-212 Log in to see Polyclonal
0.0010115753 ABIN4312611 ICC IHC (p) Rabbit N-Term Log in to see Polyclonal
0.0010115753 ABIN4312612 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
0.0010115753 ABIN2765210 ICC IHC (p) IHC Rabbit N-Term Log in to see Polyclonal

Similar anti-FZD6 Antibodies

Application / Reactivity Human
ELISA 31 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
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Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 34 Antibodies

General

Antigen Frizzled Family Receptor 6 (FZD6) Antibodies
Epitope N-Term, Extracellular Domain
(18), (15), (13), (10), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(62), (16), (7), (5), (1)
Host Rabbit
(61), (4), (4), (1)
Clonality
Polyclonal
Conjugate This FZD6 antibody is un-conjugated
(4), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(42), (35), (14), (13), (9), (5), (4), (2), (1), (1), (1), (1), (1)
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Product Details anti-FZD6 Antibody

Target Details FZD6 Application Details Handling Images
Specificity Human Frizzled-6. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD3 (50 %).
Cross-Reactivity Monkey
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Mouse, Panda, Bovine, Dog, Horse, Rabbit, Pig (94%) Rat, Opossum, Turkey, Chicken (88%) Elephant, Bat, Lizard (81%).
Purification Immunoaffinity Chromatography
Immunogen Synthetic 16 amino acid peptide from N-terminal extracellular domain of human Frizzled-6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Panda, Bovine, Dog, Horse, Rabbit, Pig (94%), Rat, Opossum, Turkey, Chicken (88%), Elephant, Bat, Lizard (81%).
ImmunogenType: Synthetic peptide

Target Details FZD6

Product Details anti-FZD6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name FZD6 / Frizzled 6 (FZD6 Antibody Abstract)
Background Standard Gene Symbol: FZD6, Gene Family: GPCR, Gene Subfamily: Frizzled, Synonyms: FZD6, Frizzled family receptor 6, FZ-6, HFZ6, Frizzled 6, Frizzled-6, Frizzled homolog 6, FZ6, NDNC10
Gene ID 8323
Pathways WNT Signaling

Application Details

Product Details anti-FZD6 Antibody Target Details FZD6 Handling Images back to top
Application Notes ICC (3 - 6 μg/mL), IHC-P (6 - 14 μg/mL),
Comment

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-FZD6 Antibody Target Details FZD6 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-80 °C
Storage Comment Long term: -70°C, Short term: +4°C

Images

Product Details anti-FZD6 Antibody Target Details FZD6 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-FZD6 antibody (Frizzled Family Receptor 6) (N-Term) (ABIN1048614) Anti-Frizzled-6 antibody ABIN1048614 IHC staining of human pancreas. Immunohistochem...