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anti-Human FZD6 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended FZD6 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 6 (FZD6) Antibodies
  • zgc:65879
  • zgc:77440
  • fz6
  • frz6
  • Xfrz6
  • frizzled6
  • frizzled-6
  • FZ-6
  • FZ6
  • HFZ6
  • NDNC10
  • Frizzled-6
  • Fz6
  • frizzled homolog 6 (Drosophila)
  • frizzled family receptor 6
  • frizzled 6
  • fzd6
  • LOC100344394
  • FZD6
  • Fzd6
Epitope
N-Term
18
15
13
11
4
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
65
18
7
5
1
Host
Rabbit
63
6
4
1
Clonality
Polyclonal
Conjugate
This FZD6 antibody is un-conjugated
4
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
45
35
15
14
10
6
4
4
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN271086
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.203815 ABIN1713791 IF (p) IHC (p) WB Rabbit IgG AA 19-50 Log in to see Polyclonal
11.203815 ABIN4312612 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
11.203815 ABIN4312611 ICC IHC (p) Rabbit N-Term Log in to see Polyclonal
1 ABIN122081 IHC (fro) IF IHC (p) Rabbit N-Term Log in to see Polyclonal
1 ABIN1048614 ICC IHC (p) Rabbit N-Term, Extracellular Domain Log in to see Polyclonal
1 ABIN1048613 IHC (p) Rabbit N-Term, Extracellular Domain Log in to see Polyclonal
1 ABIN1734469 IHC (p) Rabbit IgG N-Term, Extracellular Domain Log in to see Polyclonal
1 ABIN1734470 ICC IHC (p) Rabbit IgG N-Term, Extracellular Domain Log in to see Polyclonal
1 ABIN2765210 ICC IHC (p) IHC Rabbit N-Term Log in to see Polyclonal
1 ABIN2765211 IHC (p) Rabbit N-Term Log in to see Polyclonal

Similar anti-FZD6 Antibodies

Application / Reactivity Human
ELISA 31 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 36 Antibodies

General

Antigen Frizzled Family Receptor 6 (FZD6) Antibodies
Epitope N-Term
(18), (15), (13), (11), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(65), (18), (7), (5), (1)
Host Rabbit
(63), (6), (4), (1)
Clonality
Polyclonal
Conjugate This FZD6 antibody is un-conjugated
(4), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(45), (35), (15), (14), (10), (6), (4), (4), (1), (1), (1), (1), (1)
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Product Details anti-FZD6 Antibody

Target Details FZD6 Application Details Handling Images
Specificity Human Frizzled-6. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MTMR10 (47 % ).
Cross-Reactivity (Details) There is 76% identity,88% homology to mouse. There is 0% identity/homology with rat. Expected to react with the following species based on sequence Identity: Gorilla, Orangutan, Gibbon, Monkey, Marmoset, (94%), Elephant, Pig, Opossum (88%), Panda, Bat, Rabbit (82%).
Purification Immunogen affinity purified
Immunogen Synthetic 17 amino acid peptide from N-terminal extracellular domain of human Frizzled-6.

Target Details FZD6

Product Details anti-FZD6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Frizzled-6 (FZD6 Antibody Abstract)
Background Gene Symbol: FZD6
Gene ID 8323
UniProt O60353
Pathways WNT Signaling

Application Details

Product Details anti-FZD6 Antibody Target Details FZD6 Handling Images back to top
Application Notes Immunohistochemistry-Paraffin 14 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Frizzled-6 Antibody (NLS4481)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Restrictions For Research Use only

Handling

Product Details anti-FZD6 Antibody Target Details FZD6 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-FZD6 Antibody Target Details FZD6 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-FZD6 antibody (Frizzled Family Receptor 6) (N-Term) (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-FZD6 antibody (Frizzled Family Receptor 6) (N-Term) (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-FZD6 antibody (Frizzled Family Receptor 6) (N-Term) (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-FZD6 antibody (Frizzled Family Receptor 6) (N-Term) (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-FZD6 antibody (Frizzled Family Receptor 6) (N-Term) (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...