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|Application / Reactivity||Mouse (Murine)|
|Flow Cytometry (FACS)||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||1 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||1 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||14 Antibodies|
|Antigen||Frizzled Family Receptor 6 (FZD6) Antibodies|
|Epitope||AA 19-153 Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This FZD6 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-FZD6 AntibodyTarget Details FZD6 Application Details Handling References for anti-FZD6 Antibody (ABIN4899379) Images
|Specificity||Detects mouse Frizzled-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10 % cross-reactivity with recombinant human Frizzled-6 is observed, and less than 1 % cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8, and rmFrizzled-9 is observed.|
|Purification||Immunogen affinity purified|
|Sterility||0.2 μm filtered|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-6, His19-Val153, Accession # Q61089|
Target Details FZD6Product Details anti-FZD6 Antibody Application Details Handling References for anti-FZD6 Antibody (ABIN4899379) Images back to top
|Alternative Name||Frizzled-6 (FZD6 Antibody Abstract)|
Application DetailsProduct Details anti-FZD6 Antibody Target Details FZD6 Handling References for anti-FZD6 Antibody (ABIN4899379) Images back to top
|Application Notes||Western Blot 0.1 μg/mL, Flow Cytometry 2.5 μg/10^6 cells, Immunohistochemistry 5-15 μg/mL, CyTOF-ready|
|Restrictions||For Research Use only|
HandlingProduct Details anti-FZD6 Antibody Target Details FZD6 Application Details References for anti-FZD6 Antibody (ABIN4899379) Images back to top
|Reconstitution||Reconstitute at 0.2 mg/mL in sterile PBS.|
|Buffer||0.2 m filtered solution in PBS with Trehalose.|
|Storage||4 °C/-20 °C/-80 °C|
|Storage Comment||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.|
|Expiry Date||12 months|
References for anti-FZD6 Antibody (ABIN4899379)Product Details anti-FZD6 Antibody Target Details FZD6 Application Details Handling Images back to top
|Product cited in:||
Chang, Smallwood, Williams, Nathans: "The spatio-temporal domains of Frizzled6 action in planar polarity control of hair follicle orientation." in: Developmental biology, Vol. 409, Issue 1, pp. 181-93, 2015 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).
Lau, Tao, Liu, Wen, Sturgeon, Sorfazlian, Lazic, Burrows, Wong, Li, Deimling, Ciruna, Scott, Simmons, Henkelman, Williams, Hadjantonakis, Fernandez-Gonzalez, Sun, Hopyan: "Anisotropic stress orients remodelling of mammalian limb bud ectoderm." in: Nature cell biology, Vol. 17, Issue 5, pp. 569-79, 2015 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).
Li, Xu, Li, Li, Bai, Strauss, Zhang, Wang: "Otitis media in sperm-associated antigen 6 (Spag6)-deficient mice." in: PLoS ONE, Vol. 9, Issue 11, pp. e112879, 2014 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).
Webb, Grillet, Andrade, Xiong, Swarthout, Della Santina, Kachar, Müller: "Regulation of PCDH15 function in mechanosensory hair cells by alternative splicing of the cytoplasmic domain." in: Development (Cambridge, England), Vol. 138, Issue 8, pp. 1607-17, 2011 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).