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anti-Human FZD8 antibody for Western Blotting

Recommended FZD8 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 8 (FZD8) Antibodies
  • cFz-8
  • frizzled-8
  • frizzled8
  • Fz-8
  • FZ-8
  • Fz8
  • fzd8
  • FZD8
  • hFZ8
  • hfz8
  • xfz8
  • Xfz8
Epitope
N-Term
18
13
7
3
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
57
26
14
3
3
1
1
Host
Rabbit
41
20
3
1
Clonality
Polyclonal
Conjugate
This FZD8 antibody is un-conjugated
3
1
1
1
1
1
Application
Western Blotting (WB)
45
34
23
15
5
1
1
Supplier
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Catalog No. ABIN2776728
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN1535615 IF ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1535685 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN2855896 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.12303223 ABIN2704804 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN2465732 IHC ELISA WB Goat C-Term Log in to see Polyclonal
0.12303223 ABIN250857 ICC IF IHC (p) IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal
0.12303223 ABIN2439910 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2439912 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN635838 WB Rabbit Log in to see Polyclonal
0.12303223 ABIN2435843 IHC WB Goat Log in to see Polyclonal
0.12303223 ABIN1852954 ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011806369 ABIN185522 IHC ELISA WB Goat C-Term Log in to see Polyclonal 1
0.0011806369 ABIN374634 IHC (p) WB Goat C-Term Log in to see Polyclonal
0.0011806369 ABIN563593 ELISA WB Mouse AA 72-161, partial Log in to see Polyclonal 1
0.0011806369 ABIN317702 IHC (p) WB Rabbit Log in to see Polyclonal
0.0011806369 ABIN468712 WB Rabbit N-Term Log in to see Polyclonal
0.0011806369 ABIN297075 ELISA WB Goat C-Term Log in to see Polyclonal
0.0011806369 ABIN2890828 ELISA WB Rabbit IgG AA 486-535 Log in to see Polyclonal
0.0011806369 ABIN2890784 IF ELISA WB Rabbit IgG AA 31-80 Log in to see Polyclonal
0.0011806369 ABIN1455216 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-FZD8 antibody for Western Blotting

Similar anti-FZD8 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 40 Antibodies 23 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies 6 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 9 Antibodies 6 Antibodies
Immunofluorescence (IF) 6 Antibodies 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Frizzled Family Receptor 8 (FZD8) Antibodies
Epitope N-Term
(18), (13), (7), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(57), (26), (14), (3), (3), (1), (1)
Host Rabbit
(41), (20), (3), (1)
Clonality
Polyclonal
Conjugate This FZD8 antibody is un-conjugated
(3), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(45), (34), (23), (15), (5), (1), (1)
Pubmed 2 references available
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Product Details anti-FZD8 Antibody

Target Details FZD8 Application Details Handling References for anti-FZD8 Antibody (ABIN2776728) Images
Blocking Peptide Blocking peptide for this product available: ABIN977174
Sequence PDTLCMDYNR TDLTTAAPSP PRRLPPPPPG EQPPSGSGHG RPPGARPPHR
Predicted Reactivity Human : 100 %, Mouse : 90 %, Rat : 90 %
Characteristics This is a rabbit polyclonal antibody against FZD8. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human FZD8

Target Details FZD8

Product Details anti-FZD8 Antibody Application Details Handling References for anti-FZD8 Antibody (ABIN2776728) Images back to top
Antigen
Alternative Name FZD8 (FZD8 Antibody Abstract)
Background FZD8 is a member of the frizzled family. Members of this family are seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.
Molecular Weight 73 kDa
Gene ID 8325
NCBI Accession NP_114072, NM_031866
UniProt Q9H461
Pathways WNT Signaling

Application Details

Product Details anti-FZD8 Antibody Target Details FZD8 Handling References for anti-FZD8 Antibody (ABIN2776728) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 694 AA

Restrictions For Research Use only

Handling

Product Details anti-FZD8 Antibody Target Details FZD8 Application Details References for anti-FZD8 Antibody (ABIN2776728) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FZD8 Antibody (ABIN2776728)

Product Details anti-FZD8 Antibody Target Details FZD8 Application Details Handling Images back to top
Product cited in:

Yin, Xu, Bonfil, Banerjee, Sarkar, Sethi, Reddy: "Tumor-initiating cells and FZD8 play a major role in drug resistance in triple-negative breast cancer." in: Molecular cancer therapeutics, Vol. 12, Issue 4, pp. 491-8, 2013

Background publications

Lu, Yamamoto, Ortega, Baltimore: "Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth." in: Cell, Vol. 119, Issue 1, pp. 97-108, 2004

Images

Product Details anti-FZD8 Antibody Target Details FZD8 Application Details Handling References for anti-FZD8 Antibody (ABIN2776728) back to top
Supplier Images
Western Blotting (WB) image for anti-FZD8 antibody (Frizzled Family Receptor 8) (N-Term) (ABIN2776728) WB Suggested Anti-FZD8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...