Mouse (Murine) IGFBP6 ELISA Kit

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Antigen
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA Kits
  • IGFBP-6
  • IGFBP6
  • igfbp6
  • IBP6
  • IGF-BP6
  • RBP6A
  • insulin-like growth factor binding protein 6
  • insulin-like growth factor binding protein-6
  • IGFBP-6
  • IGFBP6
  • igfbp6
  • Igfbp6
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
18
12
11
7
4
3
2
2
2
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
6-6000 pg/mL
Minimum Detection Limit
6 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625132
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.214675 ABIN1115549 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
10.214675 ABIN1570553 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 6.25-400 ng/mL 6.25 ng/mL Log in to see
1 ABIN365249 Sandwich ELISA Serum, Plasma, Tissue Homogenate 5000-78.13 pg/mL 5000 pg/mL Log in to see
1 ABIN4993105 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 8.2-6000 pg/mL 8.2 pg/mL Log in to see
1 ABIN628016 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 1.0-25 ng/mL 1.0 ng/mL Log in to see
1 ABIN5523446 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN4970216 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 6.25-400 ng/mL 6.25 ng/mL Log in to see
1 ABIN2948558 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN2116044 Log in to see
1 ABIN455456 Log in to see
1 ABIN5072727 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

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General

Antigen Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(18), (12), (11), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 6-6000 pg/mL
Minimum Detection Limit 6 pg/mL
Application ELISA
Supplier Log in to see

Product Details IGFBP6 ELISA Kit

Target details Application Details Handling Images
Purpose Mouse IGFBP-6 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Serum, Plasma, Cell Culture Supernatant
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivity < 6 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IGFBP6 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IGFBP-6 (IGFBP6 ELISA Kit Abstract)
Background The Mouse IGF-BP-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF-BP-6 in serum, plasma and cell culture supernates. This assay employs an antibody specific for mouse IGF-BP-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-BP-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF-BP-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-BP-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 16012
UniProt P47880
Pathways WNT Signaling, Myometrial Relaxation and Contraction

Application Details

Product Details IGFBP6 ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples5 - 50 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernates. Suggested dilution for normal serum/plasma: 5-50 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 80 µL IGF-BP-6 standard from the vial of Item C, into a tube with 586.7 µL Assay Diluent A or 1x Assay Diluent B to prepare a 6,000 pg/mL stock standard solution. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL) 200 µL 80 µL standard + 586.7 µL 200myl 200 µL 200 µL 200 µL 200 µL 6,000 2,000 666.7 222.2 74.07 24.69 8.23 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 65-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) before use. HRP-Streptavidin concentrate should be diluted 80-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 150 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare 80 fold diluted HRP- Streptavidin solution.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Mouse IGFBP-6 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Mouse IGFBP-6 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.001 0.01 0.1 1 10 .
Sensitivity: The minimum detectable dose of IGF-BP-6 is typically less than 6 pg/mL.
Recovery: Recovery was determined by spiking various levels of recombinant mouse IGF-BP-6 into normal mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 89.43 81-99 Plasma 90.76 82-101 Cell culture media 93.12 83-103
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 95 96 94 Range ( %) 85-103 84-105 84-106 1:4 Average % of Expected 94 95 96 Range ( %) 84-105 83-103 85-104
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details IGFBP6 ELISA Kit Target details Application Details Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details IGFBP6 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for IGFBP6 ELISA Kit (Insulin-Like Growth Factor Binding Protein 6) (ABIN625132) Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA Kit