anti-Human PKC alpha antibody for Enzyme Immunoassay

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C, alpha
  • PRKCA
  • prkca
  • LOC100219195
  • Prkca
Epitope
N-Term
45
26
17
16
16
15
13
12
7
6
6
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
262
188
149
29
25
23
17
17
16
5
4
3
3
3
2
1
1
1
Host
Rabbit
227
43
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
10
8
7
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
216
117
94
62
48
39
27
26
21
19
5
4
3
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN453789
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN359058 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
1 ABIN272065 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN303443 EIA IHC (p) Rabbit AA 491-501, pThr497 Log in to see Polyclonal

Top referenced anti-PKC alpha antibody for Enzyme Immunoassay

Similar anti-PKC alpha Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 70 Antibodies 111 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 33 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 59 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 23 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 146 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope N-Term
(45), (26), (17), (16), (16), (15), (13), (12), (7), (6), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(262), (188), (149), (29), (25), (23), (17), (17), (16), (5), (4), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(227), (43), (2)
Clonality
Polyclonal
Conjugate This PKC alpha antibody is un-conjugated
(11), (10), (8), (7), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(216), (117), (94), (62), (48), (39), (27), (26), (21), (19), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling ProductDetails: References for anti-PKC alpha antibody (ABIN453789) Images
Specificity This antibody reacts to PRKCA.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Immunogen KLH conjugated synthetic peptide selected from the N-terminal region of human PRKCA

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling ProductDetails: References for anti-PKC alpha antibody (ABIN453789) Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type
Gene ID 5578
NCBI Accession NP_002728
UniProt P17252
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling ProductDetails: References for anti-PKC alpha antibody (ABIN453789) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details ProductDetails: References for anti-PKC alpha antibody (ABIN453789) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-PKC alpha antibody (ABIN453789)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Background publications

Gysin, Imber: "Replacement of Ser657 of protein kinase C-alpha by alanine leads to premature down regulation after phorbol-ester-induced translocation to the membrane." in: European journal of biochemistry / FEBS, Vol. 240, Issue 3, pp. 747-50, 1996

Rider, Vandamme, Lebeau, Vertommen, Vidal, Rousseau, Vandekerckhove, Hue: "The two forms of bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase result from alternative splicing." in: The Biochemical journal, Vol. 285 ( Pt 2), pp. 405-11, 1992

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling ProductDetails: References for anti-PKC alpha antibody (ABIN453789) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (N-Term) (ABIN453789) Western blot analysis of PRKCA Antibody (N-term) in NIH-3T3 cell line lysates (35ug/l...