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anti-Human PKC alpha antibody for Enzyme Immunoassay

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • AAG6
  • AI875142
  • im:7139045
  • LOC100219195
  • pkc-alpha
  • PKC-alpha
  • Pkca
  • PKCA
  • pkcalpha
  • PKRCA
  • PRKACA
  • si:dkey-8l19.1
Epitope
C-Term
48
34
17
15
15
13
11
10
7
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
228
115
92
8
4
4
3
3
2
1
1
1
1
1
Host
Rabbit
213
27
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
10
9
7
6
5
5
4
4
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
185
107
90
34
33
22
22
20
6
5
4
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN359058
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000880999 ABIN453789 EIA WB Rabbit N-Term Log in to see Polyclonal 2
0.000880999 ABIN272065 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.000880999 ABIN303443 EIA IHC (p) Rabbit AA 491-501, pThr497 Log in to see Polyclonal

Top referenced anti-PKC alpha antibody for Enzyme Immunoassay

Similar anti-PKC alpha Antibodies

Application / Reactivity Human
ELISA 99 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 32 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 90 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope C-Term
(48), (34), (17), (15), (15), (13), (11), (10), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(228), (115), (92), (8), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(213), (27), (2)
Clonality
Polyclonal
Conjugate This PKC alpha antibody is un-conjugated
(10), (9), (7), (6), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(185), (107), (90), (34), (33), (22), (22), (20), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN359058) Images
Specificity This antibody reacts to PKC alpha.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.
Isotype Ig

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha Antibody (ABIN359058) Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC alpha is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type
Gene ID 5578, 9606
UniProt P17252
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha Antibody (ABIN359058) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha Antibody (ABIN359058) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PKC alpha Antibody (ABIN359058)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Background publications

Rabinovitz, Tsomo, Mercurio: "Protein kinase C-alpha phosphorylation of specific serines in the connecting segment of the beta 4 integrin regulates the dynamics of type II hemidesmosomes." in: Molecular and cellular biology, Vol. 24, Issue 10, pp. 4351-60, 2004

Gong, Wei, Chowdhury, Chatterjee: "Lactosylceramide recruits PKCalpha/epsilon and phospholipase A2 to stimulate PECAM-1 expression in human monocytes and adhesion to endothelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 17, pp. 6490-5, 2004

Hermoso, Olivero, Torres, Riveros, Quest, Stutzin: "Cell volume regulation in response to hypotonicity is impaired in HeLa cells expressing a protein kinase Calpha mutant lacking kinase activity." in: The Journal of biological chemistry, Vol. 279, Issue 17, pp. 17681-9, 2004

Lorenz, Lohse, Quitterer: "Protein kinase C switches the Raf kinase inhibitor from Raf-1 to GRK-2." in: Nature, Vol. 426, Issue 6966, pp. 574-9, 2003

Petroski, Deshaies: "Context of multiubiquitin chain attachment influences the rate of Sic1 degradation." in: Molecular cell, Vol. 11, Issue 6, pp. 1435-44, 2003

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN359058) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (C-Term) (ABIN359058) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (C-Term) (ABIN359058) Western blot analysis of anti-PKCalpha Pab in placenta lysate. PKCalpha (arrow) was d...