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anti-Human PKC alpha antibody for Immunoprecipitation

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C, alpha
  • PRKCA
  • prkca
  • LOC100219195
  • Prkca
Reactivity
Cow (Bovine), Dog (Canine), Fish, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
256
184
146
28
24
22
16
16
15
5
4
3
3
3
2
1
1
1
Host
Mouse
222
42
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
10
8
7
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
210
116
93
58
45
36
26
26
20
18
5
4
3
3
2
1
1
1
1
Supplier
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Catalog No. ABIN153146
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967751 IF IP IHC WB Mouse IgG2b, kappa AA 270-427 Log in to see 3-PKCalpha 5
1 ABIN967750 IF IP IHC WB Mouse IgG2b, kappa AA 270-427 Log in to see 3-PKCalpha 6
1 ABIN256441 IP WB Mouse IgG2a kappa Log in to see PKC0103
1 ABIN1493005 IHC (p) IP WB Magnetic Particles Rabbit C-Term Log in to see Polyclonal
1 ABIN1493006 IHC (p) IP WB Sepharose Rabbit C-Term Log in to see Polyclonal
1 ABIN1822874 IP WB Rabbit IgG Ser657 Log in to see Polyclonal
1 ABIN4345802 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Mouse IgG2a Log in to see MC5
1 ABIN4904772 FACS ICC IF IHC IP WB Rabbit IgG Log in to see
1 ABIN220827 IP ELISA WB Rabbit IgG Thr641, pThr638 Log in to see Polyclonal
1 ABIN199577 Func IP Neut WB Mouse IgG1 Log in to see
1 ABIN2576934 IP ELISA WB Mouse IgG2a Log in to see MC5
1 ABIN4345815 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB PerCP Mouse IgG2a Log in to see MC5
1 ABIN870311 IP ELISA WB Rabbit Log in to see G13-R
1 ABIN870310 IP ELISA WB Rabbit Log in to see G13-R
1 ABIN331530 FACS IP IHC WB Mouse IgG2a Log in to see MC5
1 ABIN342477 IP ELISA WB Rabbit Log in to see Polyclonal
1 ABIN4345803 ELISA IHC IHC (fro) IHC (p) IP WB Rabbit IgG Log in to see G13-R
1 ABIN220833 ICC FACS IF IP WB Rabbit Log in to see Polyclonal
1 ABIN1926562 IP Rabbit IgG AA 300-350 Log in to see Polyclonal
1 ABIN1926563 IP WB Rabbit IgG AA 622-672 Log in to see Polyclonal

Top referenced anti-PKC alpha antibody for Immunoprecipitation

Similar anti-PKC alpha Antibodies

Application / Reactivity Sheep (Ovine) Rat (Rattus)
ELISA 13 Antibodies 52 Antibodies
Flow Cytometry (FACS) 15 Antibodies 18 Antibodies
Immunocytochemistry (ICC) 13 Antibodies 19 Antibodies
Immunofluorescence (IF) 13 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 3 Antibodies 41 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 12 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Fish, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(256), (184), (146), (28), (24), (22), (16), (16), (15), (5), (4), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(222), (42), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(11), (10), (8), (7), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(210), (116), (93), (58), (45), (36), (26), (26), (20), (18), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN153146) Images
Specificity This antibody is capable of detecting PKC beta as well.
Purification Protein A purified
Immunogen Purified bovine brain Protein Kinase C
Clone MC5
Isotype IgG2a

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha Antibody (ABIN153146) Images back to top
Antigen
Alternative Name PKC alpha (PKCa Antibody Abstract)
Background Gene Symbol: PRKCA
Gene ID 5578
UniProt P17252, P04409
Research Area Protein Modifications, Signaling, Kinases/Phosphatases, Cardiovascular, Hypertrophy
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha Antibody (ABIN153146) Images back to top
Application Notes Western Blot 1:1000, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:200-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunoprecipitation 1:10-1:100, Immunohistochemistry-Paraffin 1:200-1:500, Immunohistochemistry-Frozen 1:200-1:500, RadioimmunoassayThis PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha Antibody (ABIN153146) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-PKC alpha Antibody (ABIN153146)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Product cited in:

Guimarães-Souza, Perche, Morgans, Duvoisin, Calaza: "Fragile X Mental Retardation Protein expression in the retina is regulated by light." in: Experimental eye research, Vol. 146, pp. 72-82, 2016

Zimov, Yazulla: "Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells." in: Cell and tissue research, Vol. 333, Issue 1, pp. 1-16, 2008

Quraishi, Gayet, Morgans, Duvoisin: "Distribution of group-III metabotropic glutamate receptors in the retina." in: The Journal of comparative neurology, Vol. 501, Issue 6, pp. 931-43, 2007

Bayley, Morgans: "Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina." in: The Journal of comparative neurology, Vol. 500, Issue 2, pp. 286-98, 2006

Ng, Parsons, Hughes, Monypenny, Zicha, Gautreau, Arpin, Gschmeissner, Verveer, Bastiaens, Parker: "Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility." in: The EMBO journal, Vol. 20, Issue 11, pp. 2723-41, 2001 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunoprecipitation,Immunofluorescence

Cesare, Dekker, Sardini, Parker, McNaughton: "Specific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat." in: Neuron, Vol. 23, Issue 3, pp. 617-24, 1999 (Sample species: Rat (Rattus)). Further details: Western Blotting

Ricciarelli, Tasinato, Clément, Ozer, Boscoboinik, Azzi: "alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state." in: The Biochemical journal, Vol. 334 ( Pt 1), pp. 243-9, 1998 (Sample species: Rat (Rattus)). Further details: Western Blotting

Gatti, Robinson: "Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31718-22, 1997 (Sample species: Rat (Rattus)). Further details: Western Blotting

Clément, Tasinato, Boscoboinik, Azzi: "The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation." in: European journal of biochemistry, Vol. 246, Issue 3, pp. 745-9, 1997 (Sample species: Rat (Rattus)). Further details: Western Blotting

Gatti, Robinson: "Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon." in: European journal of biochemistry, Vol. 249, Issue 1, pp. 92-7, 1997 (Sample species: Rat (Rattus)). Further details: Western Blotting

Wyss-Coray, Masliah, Toggas, Rockenstein, Brooker, Lee, Mucke: "Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis." in: The Journal of clinical investigation, Vol. 97, Issue 3, pp. 789-98, 1996 Further details: Immunocytochemistry,Immunofluorescence

Escribá, Sastre, García-Sevilla: "Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7595-9, 1995 (Sample species: Rat (Rattus)). Further details: Western Blotting

Lacal, Cuadrado, Jones, Trotta, Burstein, Thomson, Pellicer: "Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells." in: Molecular and cellular biology, Vol. 10, Issue 6, pp. 2983-90, 1990 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN153146) back to top
Supplier Images
Immunofluorescence (IF) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (ABIN153146) Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) [ABIN153146] - PKC a...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (ABIN153146) Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [ABIN153146] - IHC staining o...