We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||12 Antibodies||16 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||6 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||17 Antibodies|
|Antigen||Secreted Frizzled-Related Protein 1 (SFRP1) Antibodies|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SFRP1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|8 references available|
|Supplier||Log in to see|
Product Details anti-SFRP1 AntibodyTarget Details SFRP1 Application Details Handling References for anti-SFRP1 Antibody (ABIN401251) Images
|Specificity||This affinity purified antibody is directed against Human SFRP protein. A BLAST analysis was used to suggest reactivity with this protein from Human based on 100 % homology for the immunogen sequence. Expect cross reactivity with SFRP1 from Mouse, Rat and Bovine sources as only a single amino acid residue change is found within the immunogen sequence (91 % positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.|
Species reactivity (expected):Mouse, Rat and Bovine.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to a 12 amino acid region of Human Sfrp1 protein|
Target Details SFRP1Product Details anti-SFRP1 Antibody Application Details Handling References for anti-SFRP1 Antibody (ABIN401251) Images back to top
|Alternative Name||SFRP1 (SFRP1 Antibody Abstract)|
|Background||SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.Synonyms: FRP, FRP-1, FRP1, SARP-2, SARP2, SFRP-1, Secreted apoptosis-related protein 2, Secreted frizzled-related protein 1|
|Molecular Weight||35.4 kDa (Predicted)|
|Research Area||Organogenesis, Wnt, Proteolysis / Ubiquitin, Proteasome, Wnt Signaling|
Application DetailsProduct Details anti-SFRP1 Antibody Target Details SFRP1 Handling References for anti-SFRP1 Antibody (ABIN401251) Images back to top
ELISA: 1: 5,000 - 1: 25,000. Immunohistochemistry on paraffin sections: 1: 800. Western blot: 1: 200 - 1: 2,000 (Expect a band approximately 37 kDa in size corresponding toSfrp1 in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SFRP1 Antibody Target Details SFRP1 Application Details References for anti-SFRP1 Antibody (ABIN401251) Images back to top
|Concentration||0.56 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-SFRP1 Antibody (ABIN401251)Product Details anti-SFRP1 Antibody Target Details SFRP1 Application Details Handling Images back to top
Garcia-Hoyos, Cantalapiedra, Arroyo et al.: "Evaluation of SFRP1 as a candidate for human retinal dystrophies." in: Molecular vision, Vol. 10, pp. 426-31, 2004 (PubMed).
Han, Amar: "Secreted frizzled-related protein 1 (SFRP1) protects fibroblasts from ceramide-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2832-40, 2004 (PubMed).
Ko, Ryu, Lee et al.: "Human secreted frizzled-related protein is down-regulated and induces apoptosis in human cervical cancer." in: Experimental cell research, Vol. 280, Issue 2, pp. 280-7, 2002 (PubMed).
Chong, Uren, Rubin et al.: "Disulfide bond assignments of secreted Frizzled-related protein-1 provide insights about Frizzled homology and netrin modules." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 5134-44, 2002 (PubMed).
Uren, Reichsman, Anest et al.: "Secreted frizzled-related protein-1 binds directly to Wingless and is a biphasic modulator of Wnt signaling." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4374-82, 2000 (PubMed).
Melkonyan, Chang, Shapiro et al.: "SARPs: a family of secreted apoptosis-related proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 25, pp. 13636-41, 1998 (PubMed).
Finch, He, Kelley et al.: "Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 13, pp. 6770-5, 1997 (PubMed).
Rattner, Hsieh, Smallwood et al.: "A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 7, pp. 2859-63, 1997 (PubMed).
ImagesProduct Details anti-SFRP1 Antibody Target Details SFRP1 Application Details Handling References for anti-SFRP1 Antibody (ABIN401251) back to top