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anti-Mouse (Murine) SFRP1 antibody for Immunofluorescence

Recommended SFRP1 Antibody (supplied by: Log in to see )

Antigen
Secreted Frizzled-Related Protein 1 (SFRP1) Antibodies
  • 2210415K03Rik
  • AW011917
  • AW107218
  • AW742929
  • CSFRP1
  • FRP
  • frp
  • FRP-1
  • frp-1
  • frp1
  • FRP1
  • frzA
  • FrzA
  • LOC100231868
  • sarp2
  • SARP2
  • sFRP-1
  • sfrp1
  • SFRP1
  • XfrzA
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
87
39
32
9
3
2
1
1
Host
Rabbit
75
24
2
Clonality
Polyclonal
Conjugate
This SFRP1 antibody is un-conjugated
9
6
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
62
55
30
25
12
8
5
4
3
3
2
1
1
Supplier
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Catalog No. ABIN401251
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843398 ABIN303265 EIA IF IHC (p) WB Rabbit AA 43-54 Log in to see Polyclonal
0.0010843398 ABIN2470303 IF IHC ELISA WB Rabbit AA 43-54 Log in to see Polyclonal
0.0010843398 ABIN1739218 IF IHC (p) ELISA WB Rabbit IgG AA 43-54 Log in to see Polyclonal
0.0010843398 ABIN4353133 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0010843398 ABIN2502520 IF IHC ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-SFRP1 antibody for Immunofluorescence

Similar anti-SFRP1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 12 Antibodies 17 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 3 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 2 Antibodies
Immunofluorescence (IF) 3 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 17 Antibodies

General

Antigen Secreted Frizzled-Related Protein 1 (SFRP1) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(87), (39), (32), (9), (3), (2), (1), (1)
Host Rabbit
(75), (24), (2)
Clonality
Polyclonal
Conjugate This SFRP1 antibody is un-conjugated
(9), (6), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(62), (55), (30), (25), (12), (8), (5), (4), (3), (3), (2), (1), (1)
Pubmed 8 references available
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Product Details anti-SFRP1 Antibody

Target Details SFRP1 Application Details Handling References for anti-SFRP1 Antibody (ABIN401251) Images
Specificity This affinity purified antibody is directed against Human SFRP protein. A BLAST analysis was used to suggest reactivity with this protein from Human based on 100 % homology for the immunogen sequence. Expect cross reactivity with SFRP1 from Mouse, Rat and Bovine sources as only a single amino acid residue change is found within the immunogen sequence (91 % positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat and Bovine.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography
Immunogen Synthetic peptide corresponding to a 12 amino acid region of Human Sfrp1 protein
Isotype IgG

Target Details SFRP1

Product Details anti-SFRP1 Antibody Application Details Handling References for anti-SFRP1 Antibody (ABIN401251) Images back to top
Antigen
Alternative Name SFRP1 (SFRP1 Antibody Abstract)
Background SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.Synonyms: FRP, FRP-1, FRP1, SARP-2, SARP2, SFRP-1, Secreted apoptosis-related protein 2, Secreted frizzled-related protein 1
Molecular Weight 35.4 kDa (Predicted)
Gene ID 6422
NCBI Accession NP_003003
UniProt Q8N474
Research Area Organogenesis, Wnt, Proteolysis / Ubiquitin, Proteasome, Wnt Signaling
Pathways WNT Signaling

Application Details

Product Details anti-SFRP1 Antibody Target Details SFRP1 Handling References for anti-SFRP1 Antibody (ABIN401251) Images back to top
Application Notes ELISA: 1: 5,000 - 1: 25,000. Immunohistochemistry on paraffin sections: 1: 800. Western blot: 1: 200 - 1: 2,000 (Expect a band approximately 37 kDa in size corresponding toSfrp1 in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SFRP1 Antibody Target Details SFRP1 Application Details References for anti-SFRP1 Antibody (ABIN401251) Images back to top
Concentration 0.56 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-SFRP1 Antibody (ABIN401251)

Product Details anti-SFRP1 Antibody Target Details SFRP1 Application Details Handling Images back to top
Background publications

Garcia-Hoyos, Cantalapiedra, Arroyo, Esteve, Rodríguez, Riveiro, Trujillo, Ramos, Bovolenta, Ayuso: "Evaluation of SFRP1 as a candidate for human retinal dystrophies." in: Molecular vision, Vol. 10, pp. 426-31, 2004

Han, Amar: "Secreted frizzled-related protein 1 (SFRP1) protects fibroblasts from ceramide-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2832-40, 2004

Ko, Ryu, Lee, Na, Kim, Oh, Kim, Kim: "Human secreted frizzled-related protein is down-regulated and induces apoptosis in human cervical cancer." in: Experimental cell research, Vol. 280, Issue 2, pp. 280-7, 2002

Chong, Uren, Rubin, Speicher: "Disulfide bond assignments of secreted Frizzled-related protein-1 provide insights about Frizzled homology and netrin modules." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 5134-44, 2002

Uren, Reichsman, Anest, Taylor, Muraiso, Bottaro, Cumberledge, Rubin: "Secreted frizzled-related protein-1 binds directly to Wingless and is a biphasic modulator of Wnt signaling." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4374-82, 2000

Melkonyan, Chang, Shapiro, Mahadevappa, Fitzpatrick, Kiefer, Tomei, Umansky: "SARPs: a family of secreted apoptosis-related proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 25, pp. 13636-41, 1998

Finch, He, Kelley, Uren, Schaudies, Popescu, Rudikoff, Aaronson, Varmus, Rubin: "Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 13, pp. 6770-5, 1997

Rattner, Hsieh, Smallwood, Gilbert, Copeland, Jenkins, Nathans: "A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 7, pp. 2859-63, 1997

Images

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Supplier Images
 image for anti-SFRP1 antibody (Secreted Frizzled-Related Protein 1) (ABIN401251) anti-Secreted Frizzled-Related Protein 1 (SFRP1) antibody
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