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anti-Human SFRP2 antibody for Enzyme Immunoassay

Recommended SFRP2 Antibody (supplied by: Log in to see )

Antigen
Secreted Frizzled-Related Protein 2 (SFRP2) Antibodies
  • AI851596
  • FRP-2
  • frp-2
  • hm:zeh0225
  • SARP1
  • sarp1
  • SDF-5
  • sdf-5
  • Sdf5
  • SFRP-2
  • sfrp-2
  • SFRP2
  • sfrp2
  • zeh0225
  • zgc:153618
Epitope
C-Term
13
12
4
3
3
3
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
58
22
20
8
2
2
1
1
1
1
Host
Rabbit
40
15
3
Clonality
Polyclonal
Conjugate
This SFRP2 antibody is un-conjugated
3
3
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
37
28
11
2
2
2
1
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Catalog No. ABIN499108
$ 456.50
Plus shipping costs $45.00

Top referenced anti-SFRP2 antibody for Enzyme Immunoassay

Similar anti-SFRP2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 39 Antibodies 21 Antibodies 19 Antibodies
Immunoprecipitation (IP) 2 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 2 Antibodies 1 Antibodies

General

Antigen Secreted Frizzled-Related Protein 2 (SFRP2) Antibodies
Epitope C-Term
(13), (12), (4), (3), (3), (3), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(58), (22), (20), (8), (2), (2), (1), (1), (1), (1)
Host Rabbit
(40), (15), (3)
Clonality
Polyclonal
Conjugate This SFRP2 antibody is un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(37), (28), (11), (2), (2), (2), (1)
Pubmed 8 references available
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Product Details anti-SFRP2 Antibody

Target Details SFRP2 Application Details Handling References for anti-SFRP2 Antibody (ABIN499108) Images
Specificity This antibody will label ~35.4 kDa protein in Western blot positive control for Sfrp2 and several other tissues.
Purification Immobilized-Antigen Affinity Chromatography
Immunogen Synthetic peptide corresponding to unique epitope on SFRP2. The peptide sequence was selected from C-terminal, amimo acids 250-295. .Remarks: The SFRP2 peptide was post-synthetically covalently modified to achieve desired antigenicity

Target Details SFRP2

Product Details anti-SFRP2 Antibody Application Details Handling References for anti-SFRP2 Antibody (ABIN499108) Images back to top
Antigen
Alternative Name SFRP2 (SFRP2 Antibody Abstract)
Background Secreted frizziled-related protein 2 (Sfrp2) is a member of Sfrp family of proteins, consisting of five members in mammals. The N-terminal region of these proteins shares sequence homology with extracellular Wnt binding domain of frizziled receptors (1). Wnts are a large family of cysteine-rich secreted glycoproteins that control development in various organisms (2). Since Sfrp family of proteins interact directly with Wnts, the Sfrp family of proteins have also been implicated in early embryonic development as well as modulating apoptotic events (3). More specifically, Sfrp2 has been shown to play a role in regulating cell growth and differentiation and it is an essential protein required in eye retinal development and myogenesis (4). Sfrp2 is also known to be a tumor-suppressor gene which is epigenetically silenced in medulloblastoma (5). Recently it was reported that Sfrp2 is a novel stimulator of angiogenesis (6). Normal expression of this protein stimulates angiogenesis via a calcineurin/NFAT pathway whereas aberrant expression of this protein is usually found in the vasculature of majority of human breast tumors (7). While this protein is overexpressed in breast tumors, it is downregulated in human cervical cancers through promoter hypermethylation (8). This downregulation leads to abnormal activation of Wnt/β-catenin signaling pathway which can be attenuated by restoring the expression of SFRP2, hence suppressing cancer cell growth (8). Sfrp2 is located on human chromosome 4q31.3 and is highly expressed in adipose tissue, intestine and colon. It also expressed in heart, brain, skeletal muscle, pancreas, thymus, prostate, testis and ovary. Sfrp2 is approximately a 35.4 kDa protein (295 amino acids).Synonyms: FKSG12, FRP-2, FRP2, Frizzled-related protein 2, SARP-1, SARP1, SFRP-2, Secreted apoptosis-related protein 1, Secreted frizzled-related protein 2
Gene ID 6423
NCBI Accession NP_003004
UniProt Q96HF1
Research Area Cardiovascular, Cardiogenesis, Wnt, Wnt Signaling
Pathways WNT Signaling

Application Details

Product Details anti-SFRP2 Antibody Target Details SFRP2 Handling References for anti-SFRP2 Antibody (ABIN499108) Images back to top
Application Notes Suitable for use in ELISA and Western Blot. Recommended Dilutions: ELISA: < 1/10,000Western Blot: > 1/500
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SFRP2 Antibody Target Details SFRP2 Application Details References for anti-SFRP2 Antibody (ABIN499108) Images back to top
Concentration 0.64-0.72 mg/mL
Buffer Stabilized buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.

References for anti-SFRP2 Antibody (ABIN499108)

Product Details anti-SFRP2 Antibody Target Details SFRP2 Application Details Handling Images back to top
Background publications

Silva, Yi, Hayes, Seigel, Hackam: "Lithium chloride regulates the proliferation of stem-like cells in retinoblastoma cell lines: a potential role for the canonical Wnt signaling pathway." in: Molecular vision, Vol. 16, pp. 36-45, 2010

Kongkham, Northcott, Croul, Smith, Taylor, Rutka: "The SFRP family of WNT inhibitors function as novel tumor suppressor genes epigenetically silenced in medulloblastoma." in: Oncogene, Vol. 29, Issue 20, pp. 3017-24, 2010

Ferreri, Illerhaus, Zucca, Cavalli: "Flows and flaws in primary central nervous system lymphoma." in: Nature reviews. Clinical oncology, Vol. 7, Issue 8, pp. doi:10.1038/nrclinonc.2010.9-c1; author reply doi:10:1038/nrclinonc.2010.9-c2, 2010

Courtwright, Siamakpour-Reihani, Arbiser, Banet, Hilliard, Fried, Livasy, Ketelsen, Nepal, Perou, Patterson, Klauber-Demore: "Secreted frizzle-related protein 2 stimulates angiogenesis via a calcineurin/NFAT signaling pathway." in: Cancer research, Vol. 69, Issue 11, pp. 4621-8, 2009

Chung, Lai, Sytwu, Yan, Shih, Chang, Yu, Liu, Chu, Lin: "SFRP1 and SFRP2 suppress the transformation and invasion abilities of cervical cancer cells through Wnt signal pathway." in: Gynecologic oncology, Vol. 112, Issue 3, pp. 646-53, 2009

Bhati, Patterson, Livasy, Fan, Ketelsen, Hu, Reynolds, Tanner, Moore, Gabrielli, Perou, Klauber-DeMore: "Molecular characterization of human breast tumor vascular cells." in: The American journal of pathology, Vol. 172, Issue 5, pp. 1381-90, 2008

Leyns, Bouwmeester, Kim, Piccolo, De Robertis: "Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer." in: Cell, Vol. 88, Issue 6, pp. 747-56, 1997

Wang, Krinks, Lin, Luyten, Moos: "Frzb, a secreted protein expressed in the Spemann organizer, binds and inhibits Wnt-8." in: Cell, Vol. 88, Issue 6, pp. 757-66, 1997