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anti-Human SFRP4 antibody for Immunocytochemistry

Recommended SFRP4 Antibody (supplied by: Log in to see )

Antigen
Secreted Frizzled-Related Protein 4 (SFRP4) Antibodies
  • FRP-4
  • FRPHE
  • sFRP-4
  • SFRP4
Epitope
AA 25-222
17
13
9
8
7
7
5
5
5
2
2
2
1
1
1
Reactivity
Human
79
35
25
4
4
1
1
1
1
Host
Rabbit
72
17
3
Clonality
Polyclonal
Conjugate
This SFRP4 antibody is un-conjugated
10
8
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
64
52
24
16
15
11
6
5
3
2
2
Supplier
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Available images

Catalog No. ABIN1860536
$ 385.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN1860534 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-346 Log in to see Polyclonal
0.0009962841 ABIN3201774 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 265-346 Log in to see
0.0009962841 ABIN1176502 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 265-346 Log in to see Polyclonal
0.0009962841 ABIN2969168 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-SFRP4 Antibodies

Application / Reactivity Human
ELISA 45 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Western Blotting (WB) 60 Antibodies

General

Antigen Secreted Frizzled-Related Protein 4 (SFRP4) Antibodies
Epitope AA 25-222
(17), (13), (9), (8), (7), (7), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Reactivity Human
(79), (35), (25), (4), (4), (1), (1), (1), (1)
Host Rabbit
(72), (17), (3)
Clonality
Polyclonal
Conjugate This SFRP4 antibody is un-conjugated
(10), (8), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(64), (52), (24), (16), (15), (11), (6), (5), (3), (2), (2)
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Product Details anti-SFRP4 Antibody

Target Details SFRP4 Application Details Handling Images
Sequence MGHHHHHHSG SEF-EAVRIP MCRHMPWNIT RMPNHLHHST QENAILAIEQ YEELVDVNCS AVLRFFLCAM YAPICTLEFL HDPIKPCKSV CQRARDDCEP LMKMYNHSWP ESLACDELPV YDRGVCISPE AIVTDLPEDV KWIDITPDMM VQERPLDVDC KRLSPDRCKC KKVKPTLATY LSKNYSYVIH AKIKAVQRSG CNEVTTVVDV KE
Specificity It has been selected for its ability to recognize SFRP4 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen SFRP4 (AA 25-222)
Isotype IgG

Target Details SFRP4

Product Details anti-SFRP4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Secreted Frizzled Related Protein 4 (SFRP4) (SFRP4 Antibody Abstract)
Pathways WNT Signaling

Application Details

Product Details anti-SFRP4 Antibody Target Details SFRP4 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant SFRP4 (Glu25~Glu222) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-SFRP4 Antibody Target Details SFRP4 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-SFRP4 Antibody Target Details SFRP4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-SFRP4 antibody (Secreted Frizzled-Related Protein 4) (AA 25-222) (ABIN1860536) anti-Secreted Frizzled-Related Protein 4 (SFRP4) (AA 25-222) antibody