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|Application / Reactivity||Human||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies||3 Antibodies|
|Western Blotting (WB)||68 Antibodies||26 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||12 Antibodies||6 Antibodies|
|Immunohistochemistry (IHC)||30 Antibodies||12 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunofluorescence (IF)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||2 Antibodies|
|Immunochromatography (IC)||2 Antibodies||1 Antibodies|
|Antigen||Secreted Frizzled-Related Protein 4 (SFRP4) Antibodies|
|Epitope||AA 140-346 Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This SFRP4 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-SFRP4 AntibodyTarget Details SFRP4 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Secreted frizzled-related protein 4(SFRP4) detection. Tested with WB in Human,Rat.
Gene Name: secreted frizzled-related protein 4
Protein Name: Secreted frizzled-related protein 4
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human SFRP4 recombinant protein (Position: E140-V346). Human SFRP4 shares 90% and 90.3% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively.|
Target Details SFRP4Product Details anti-SFRP4 Antibody Application Details Handling Images back to top
|Alternative Name||SFRP4 (SFRP4 Antibody Abstract)|
|Background||Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. It is mappaead to 7p14.1. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. And SFRP4 is a hub gene in a Type 2 Diabetes-associated gene coexpression module in human islets, and reduces glucose-induced insulin secretion through decreased β-cell exocytosis.|
|Research Area||Stem Cells, Wnt, Wnt Signaling|
Application DetailsProduct Details anti-SFRP4 Antibody Target Details SFRP4 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species:Human, Rat, The detection limit for SFRP4 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SFRP4 Antibody Target Details SFRP4 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-SFRP4 Antibody Target Details SFRP4 Application Details Handling back to top