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anti-Rat (Rattus) SFRP5 antibody for Enzyme Immunoassay

Recommended SFRP5 Antibody (supplied by: Log in to see )

Antigen
Secreted Frizzled-Related Protein 5 (SFRP5) Antibodies
  • AI605071
  • crescent
  • D144
  • frp1
  • frp5
  • SARP3
  • sarp3
  • sFRP-5
  • Sfrp5
  • sfrp5
  • XsFRP5
  • zgc:101849
Epitope
AA 150-200
27
22
17
7
6
5
5
3
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
44
29
10
1
Host
Rabbit
74
1
Clonality
Polyclonal
Conjugate
This SFRP5 antibody is un-conjugated
10
9
4
4
4
4
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
60
50
22
13
6
5
4
2
1
1
Supplier
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Catalog No. ABIN499109
$ 456.50
Plus shipping costs $45.00

Top referenced anti-SFRP5 antibody for Enzyme Immunoassay

Similar anti-SFRP5 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 37 Antibodies 25 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 4 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 17 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Flow Cytometry (FACS) 4 Antibodies

General

Antigen Secreted Frizzled-Related Protein 5 (SFRP5) Antibodies
Epitope AA 150-200
(27), (22), (17), (7), (6), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(44), (29), (10), (1)
Host Rabbit
(74), (1)
Clonality
Polyclonal
Conjugate This SFRP5 antibody is un-conjugated
(10), (9), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(60), (50), (22), (13), (6), (5), (4), (2), (1), (1)
Pubmed 9 references available
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Product Details anti-SFRP5 Antibody

Target Details SFRP5 Application Details Handling References for anti-SFRP5 Antibody (ABIN499109) Images
Specificity This antibody will label ~38 kDa protein in Western blot positive control for Sfrp5 and several other tissues.
Purification Immobilized-Antigen Affinity Chromatography
Immunogen Synthetic peptide corresponding to unique epitope on Sfrp5. The peptide sequence was selected from Mid-region, amimo acids 150-200. .Remarks: The SFRP5 peptide was post-synthetically covalently modified to achieve desired antigenicity.

Target Details SFRP5

Product Details anti-SFRP5 Antibody Application Details Handling References for anti-SFRP5 Antibody (ABIN499109) Images back to top
Antigen
Alternative Name SFRP5 (SFRP5 Antibody Abstract)
Background Secreted frizziled-related protein 2 (Sfrp2) is a member of Sfrp family of proteins, consisting of five members in mammals. The N-terminal region of these proteins shares sequence homology with extracellular Wnt binding domain of frizziled receptors (1). Wnts are a large family of cysteine-rich secreted glycoproteins that control development in various organisms (2). Since Sfrp family of proteins interact directly with Wnts, the Sfrp family of proteins have also been implicated in early embryonic development as well as modulating apoptotic events (3). More specifically, sfrp5 is implicated in determining the polarity of photoreceptor cells in the retina (4). Furthermore, it is an anti-inflammatory adipokine (5). Sfrp5 is a promising target in cancer treatment because it is known to play an important role in the development of bladder tumor and its downregulation or overexpression is linked with various cancers (6). Hypermethylation of the promoter region of sfrp5 deactivates this protein, this leads to oncogenic activation of wnt signaling pathway which is common in cancers (7). Therfore, inactivation of sfrp5 has been linked with oral squamous cell carcinoma, ovarian and colorectal cancers (8). Hypermethylation, as well as overexpression of sfrp5 was reported to be seen in pancreatic cancers (9). Sfrp5 is located on human chromosome 10q24.1. It is highly expressed in retinal pigment epithelium. It is also expressed in pancreas and weakly expressed in heart, liver and skeletal muscle. Sfrp5 is approximately a 38 kDa protein (317 amino acids).Synonyms: FRP-1b, FRP1B, Frizzled-related protein 1b, SARP-3, SARP3, SFRP-5, Secreted apoptosis-related protein 3, Secreted frizzled-related protein 5
Gene ID 6425
NCBI Accession NP_003006
UniProt Q5T4F7
Research Area Stem Cells, Wnt, Wnt Signaling
Pathways WNT Signaling

Application Details

Product Details anti-SFRP5 Antibody Target Details SFRP5 Handling References for anti-SFRP5 Antibody (ABIN499109) Images back to top
Application Notes Suitable for use in ELISA and Western Blot. Recommended Dilutions: ELISA: < 1/10,000Western Blot: > 1/500
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SFRP5 Antibody Target Details SFRP5 Application Details References for anti-SFRP5 Antibody (ABIN499109) Images back to top
Concentration 0.64-0.72 mg/mL
Buffer Stabilized buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.

References for anti-SFRP5 Antibody (ABIN499109)

Product Details anti-SFRP5 Antibody Target Details SFRP5 Application Details Handling Images back to top
Background publications

Ouchi, Higuchi, Ohashi, Oshima, Gokce, Shibata, Akasaki, Shimono, Walsh: "Sfrp5 is an anti-inflammatory adipokine that modulates metabolic dysfunction in obesity." in: Science (New York, N.Y.), Vol. 329, Issue 5990, pp. 454-7, 2010

Su, Lai, Lin, Liu, Chen, Chou, Lin, Lin, Lee, Yu: "Epigenetic silencing of SFRP5 is related to malignant phenotype and chemoresistance of ovarian cancer through Wnt signaling pathway." in: International journal of cancer. Journal international du cancer, Vol. 127, Issue 3, pp. 555-67, 2010

MacDonald, Tamai, He: "Wnt/beta-catenin signaling: components, mechanisms, and diseases." in: Developmental cell, Vol. 17, Issue 1, pp. 9-26, 2009

Bu, Zhao, Zhang, Gao, Lin, Dai: "Hypermethylation and aberrant expression of secreted frizzled-related protein genes in pancreatic cancer." in: World journal of gastroenterology : WJG, Vol. 14, Issue 21, pp. 3421-4, 2008

Sogabe, Suzuki, Toyota, Ogi, Imai, Nojima, Sasaki, Hiratsuka, Tokino: "Epigenetic inactivation of SFRP genes in oral squamous cell carcinoma." in: International journal of oncology, Vol. 32, Issue 6, pp. 1253-61, 2008

Urakami, Shiina, Enokida, Kawakami, Kawamoto, Hirata, Tanaka, Kikuno, Nakagawa, Igawa, Dahiya: "Combination analysis of hypermethylated Wnt-antagonist family genes as a novel epigenetic biomarker panel for bladder cancer detection." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 7 Pt 1, pp. 2109-16, 2006

Chang, Esumi, Moore, Li, Zhang, Chew, Goodman, Rattner, Moody, Stetten, Campochiaro, Zack: "Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium." in: Human molecular genetics, Vol. 8, Issue 4, pp. 575-83, 1999

Leyns, Bouwmeester, Kim, Piccolo, De Robertis: "Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer." in: Cell, Vol. 88, Issue 6, pp. 747-56, 1997

Wang, Krinks, Lin, Luyten, Moos: "Frzb, a secreted protein expressed in the Spemann organizer, binds and inhibits Wnt-8." in: Cell, Vol. 88, Issue 6, pp. 757-66, 1997