|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||36 Antibodies||24 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies||4 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies||16 Antibodies||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies|
|Antigen||Secreted Frizzled-Related Protein 5 (SFRP5) Antibodies|
|Epitope||AA 150-200 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SFRP5 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|9 references available|
|Supplier||Log in to see|
Product Details anti-SFRP5 AntibodyTarget Details SFRP5 Application Details Handling References for anti-SFRP5 Antibody (ABIN499109) Images
|Specificity||This antibody will label ~38 kDa protein in Western blot positive control for Sfrp5 and several other tissues.|
|Purification||Immobilized-Antigen Affinity Chromatography|
|Immunogen||Synthetic peptide corresponding to unique epitope on Sfrp5. The peptide sequence was selected from Mid-region, amimo acids 150-200. .Remarks: The SFRP5 peptide was post-synthetically covalently modified to achieve desired antigenicity.|
Target Details SFRP5Product Details anti-SFRP5 Antibody Application Details Handling References for anti-SFRP5 Antibody (ABIN499109) Images back to top
|Alternative Name||SFRP5 (SFRP5 Antibody Abstract)|
|Background||Secreted frizziled-related protein 2 (Sfrp2) is a member of Sfrp family of proteins, consisting of five members in mammals. The N-terminal region of these proteins shares sequence homology with extracellular Wnt binding domain of frizziled receptors (1). Wnts are a large family of cysteine-rich secreted glycoproteins that control development in various organisms (2). Since Sfrp family of proteins interact directly with Wnts, the Sfrp family of proteins have also been implicated in early embryonic development as well as modulating apoptotic events (3). More specifically, sfrp5 is implicated in determining the polarity of photoreceptor cells in the retina (4). Furthermore, it is an anti-inflammatory adipokine (5). Sfrp5 is a promising target in cancer treatment because it is known to play an important role in the development of bladder tumor and its downregulation or overexpression is linked with various cancers (6). Hypermethylation of the promoter region of sfrp5 deactivates this protein, this leads to oncogenic activation of wnt signaling pathway which is common in cancers (7). Therfore, inactivation of sfrp5 has been linked with oral squamous cell carcinoma, ovarian and colorectal cancers (8). Hypermethylation, as well as overexpression of sfrp5 was reported to be seen in pancreatic cancers (9). Sfrp5 is located on human chromosome 10q24.1. It is highly expressed in retinal pigment epithelium. It is also expressed in pancreas and weakly expressed in heart, liver and skeletal muscle. Sfrp5 is approximately a 38 kDa protein (317 amino acids).Synonyms: FRP-1b, FRP1B, Frizzled-related protein 1b, SARP-3, SARP3, SFRP-5, Secreted apoptosis-related protein 3, Secreted frizzled-related protein 5|
|Research Area||Stem Cells, Wnt, Wnt Signaling|
Application DetailsProduct Details anti-SFRP5 Antibody Target Details SFRP5 Handling References for anti-SFRP5 Antibody (ABIN499109) Images back to top
Suitable for use in ELISA and Western Blot. Recommended Dilutions: ELISA: < 1/10,000Western Blot: > 1/500
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SFRP5 Antibody Target Details SFRP5 Application Details References for anti-SFRP5 Antibody (ABIN499109) Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at -20 °C.|
References for anti-SFRP5 Antibody (ABIN499109)Product Details anti-SFRP5 Antibody Target Details SFRP5 Application Details Handling Images back to top
Ouchi, Higuchi, Ohashi et al.: "Sfrp5 is an anti-inflammatory adipokine that modulates metabolic dysfunction in obesity." in: Science (New York, N.Y.), Vol. 329, Issue 5990, pp. 454-7, 2010 (PubMed).
Su, Lai, Lin et al.: "Epigenetic silencing of SFRP5 is related to malignant phenotype and chemoresistance of ovarian cancer through Wnt signaling pathway." in: International journal of cancer. Journal international du cancer, Vol. 127, Issue 3, pp. 555-67, 2010 (PubMed).
MacDonald, Tamai, He: "Wnt/beta-catenin signaling: components, mechanisms, and diseases." in: Developmental cell, Vol. 17, Issue 1, pp. 9-26, 2009 (PubMed).
Bu, Zhao, Zhang et al.: "Hypermethylation and aberrant expression of secreted frizzled-related protein genes in pancreatic cancer." in: World journal of gastroenterology : WJG, Vol. 14, Issue 21, pp. 3421-4, 2008 (PubMed).
Sogabe, Suzuki, Toyota et al.: "Epigenetic inactivation of SFRP genes in oral squamous cell carcinoma." in: International journal of oncology, Vol. 32, Issue 6, pp. 1253-61, 2008 (PubMed).
Urakami, Shiina, Enokida et al.: "Combination analysis of hypermethylated Wnt-antagonist family genes as a novel epigenetic biomarker panel for bladder cancer detection." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 7 Pt 1, pp. 2109-16, 2006 (PubMed).
Chang, Esumi, Moore et al.: "Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium." in: Human molecular genetics, Vol. 8, Issue 4, pp. 575-83, 1999 (PubMed).
Wang, Krinks, Lin et al.: "Frzb, a secreted protein expressed in the Spemann organizer, binds and inhibits Wnt-8." in: Cell, Vol. 88, Issue 6, pp. 757-66, 1997 (PubMed).
Leyns, Bouwmeester, Kim et al.: "Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer." in: Cell, Vol. 88, Issue 6, pp. 747-56, 1997 (PubMed).