anti-Horse (Equine) WNT1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT1 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antibodies
  • Xint-1
  • Xwnt1
  • int-1
  • int1
  • wnt-1
  • wnt1-a
  • Int-1
  • Wnt-1
  • sw
  • swaying
  • BMND16
  • INT1
  • OI15
  • Int1
  • WNT-1
  • sb:eu647
  • zgc:194464
  • zgc:194478
  • WNT1
  • wingless-type MMTV integration site family, member 1
  • wingless-related MMTV integration site 1
  • WNT1
  • wnt1
  • Wnt1
  • WNT-1
Epitope
Internal Region
15
14
8
6
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
114
51
22
11
8
7
6
5
5
4
3
3
2
2
1
1
Host
Rabbit
94
20
Clonality
Polyclonal
Conjugate
This WNT1 antibody is un-conjugated
5
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
92
48
33
31
19
17
7
6
2
2
1
1
1
Supplier
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Catalog No. ABIN1049465
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1049466 IHC (p) Rabbit Internal Region Log in to see Polyclonal

General

Antigen Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antibodies
Epitope Internal Region
(15), (14), (8), (6), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(114), (51), (22), (11), (8), (7), (6), (5), (5), (4), (3), (3), (2), (2), (1), (1)
Host Rabbit
(94), (20)
Clonality
Polyclonal
Conjugate This WNT1 antibody is un-conjugated
(5), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(92), (48), (33), (31), (19), (17), (7), (6), (2), (2), (1), (1), (1)
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Product Details anti-WNT1 Antibody

Target Details WNT1 Application Details Handling Images
Brand IHC-plus™
Specificity Human WNT1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (100%) Marmoset, Hamster, Chicken, Lizard (95%) Stickleback (84%).
Purification Immunoaffinity purified
Immunogen Synthetic 19 amino acid peptide from internal region of human WNT1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (100%), Marmoset, Hamster, Chicken, Lizard (95%), Stickleback (84%).

Type of Immunogen: Synthetic peptide

Target Details WNT1

Product Details anti-WNT1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name WNT1 (WNT1 Antibody Abstract)
Background Name/Gene ID: WNT1
Family: WNT

Synonyms: WNT1, INT1, Proto-oncogene Int-1 homolog, Proto-oncogene Wnt-1
Gene ID 7471
Pathways WNT Signaling, Dopaminergic Neurogenesis

Application Details

Product Details anti-WNT1 Antibody Target Details WNT1 Handling Images back to top
Application Notes Approved: IHC-P (10 - 15 μg/mL)
Comment

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Expiry Date 12 months

Images

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Internal Region) antibody (ABIN1049465) Anti-WNT1 antibody ABIN1049465 IHC staining of human kidney. Immunohistochemistry of...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Internal Region) antibody (ABIN1049465) Anti-WNT1 antibody ABIN1049465 IHC staining of human small intestine. Immunohistoche...