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anti-Dog (Canine) WNT10B antibody for Western Blotting

Recommended WNT10B Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) Antibodies
  • SHFM6
  • wnt-12
  • WNT-12
  • wnt10
  • wnt10-A
  • WNT10B
  • wnt10br
  • Wnt12
  • wnt[d]
  • Xwnt-10
  • Xwnt-10b
  • Xwnt10b
  • Zwnt[d]
Epitope
Middle Region
13
10
7
3
3
3
2
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
56
27
20
Host
Rabbit
44
12
1
Clonality
Polyclonal
Conjugate
This WNT10B antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
35
24
23
17
11
8
4
4
3
2
Supplier
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Catalog No. ABIN2776702
$ 289.00
Plus shipping costs $45.00

Top referenced anti-WNT10B antibody for Western Blotting

Similar anti-WNT10B Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Pig (Porcine)
Western Blotting (WB) 36 Antibodies 17 Antibodies 11 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies 6 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 26 Antibodies 1 Antibodies 9 Antibodies 1 Antibodies 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunocytochemistry (ICC) 4 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) Antibodies
Epitope Middle Region
(13), (10), (7), (3), (3), (3), (2), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(56), (27), (20)
Host Rabbit
(44), (12), (1)
Clonality
Polyclonal
Conjugate This WNT10B antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(35), (24), (23), (17), (11), (8), (4), (4), (3), (2)
Pubmed 1 reference available
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Product Details anti-WNT10B Antibody

Target Details WNT10B Application Details Handling References for anti-WNT10B Antibody (ABIN2776702) Images
Blocking Peptide Blocking peptide for this product available: ABIN986210
Sequence GTSGSCQFKT CWRAAPEFRA VGAALRERLG RAIFIDTHNR NSGAFQPRLR
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 90 %
Characteristics This is a rabbit polyclonal antibody against WNT10B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human WNT10B

Target Details WNT10B

Product Details anti-WNT10B Antibody Application Details Handling References for anti-WNT10B Antibody (ABIN2776702) Images back to top
Antigen
Alternative Name WNT10B (WNT10B Antibody Abstract)
Background WNT10B is the ligand for members of the frizzled family of seven transmembrane receptors. WNT10B is a probable developmental protein. It may be a signaling molecule which affects the development of discrete regions of tissues. WNT10B is likely to signal over only few cell diameters.The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96 % identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region.
Molecular Weight 40 kDa
Gene ID 7480
NCBI Accession NM_003394, NP_003385
UniProt O00744
Pathways WNT Signaling

Application Details

Product Details anti-WNT10B Antibody Target Details WNT10B Handling References for anti-WNT10B Antibody (ABIN2776702) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 389 AA

Restrictions For Research Use only

Handling

Product Details anti-WNT10B Antibody Target Details WNT10B Application Details References for anti-WNT10B Antibody (ABIN2776702) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-WNT10B Antibody (ABIN2776702)

Product Details anti-WNT10B Antibody Target Details WNT10B Application Details Handling Images back to top
Background publications

Yoshikawa, Matsubara, Zhou, Okamura, Kubo, Murase, Shikauchi, Esteller, Herman, Wei Wang, Harris: "WNT10B functional dualism: beta-catenin/Tcf-dependent growth promotion or independent suppression with deregulated expression in cancer." in: Molecular biology of the cell, Vol. 18, Issue 11, pp. 4292-303, 2007

Images

Product Details anti-WNT10B Antibody Target Details WNT10B Application Details Handling References for anti-WNT10B Antibody (ABIN2776702) back to top
Supplier Images
Western Blotting (WB) image for anti-WNT10B antibody (Wingless-Type MMTV Integration Site Family, Member 10B) (Middle Region) (ABIN2776702) WB Suggested Anti-WNT10B Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Immunohistochemistry (IHC) image for anti-WNT10B antibody (Wingless-Type MMTV Integration Site Family, Member 10B) (Middle Region) (ABIN2776702) anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) (Middle Region) antibody (Image 2)