anti-Rat (Rattus) WNT10B antibody for ELISA

Recommended WNT10B Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) Antibodies
  • WNT10B
  • Xwnt-10b
  • Xwnt10b
  • wnt-12
  • Wnt12
  • SHFM6
  • WNT-12
  • Xwnt-10
  • wnt10
  • wnt10-A
  • Zwnt[d]
  • wnt10br
  • wnt[d]
  • wingless-type MMTV integration site family, member 10B
  • wingless related MMTV integration site 10b
  • wingless-type MMTV integration site family, member 10b
  • WNT10B
  • wnt10b
  • LOC100351316
  • Wnt10b
Reactivity
Human, Mouse (Murine), Rat (Rattus)
61
33
25
4
3
3
3
3
2
2
2
2
Host
Rabbit
47
14
1
Clonality
Polyclonal
Conjugate
This WNT10B antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
37
25
23
18
13
9
5
3
3
2
1
1
Supplier
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Catalog No. ABIN1003488
$ 466.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1031166 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 1
1 ABIN295561 IHC ELISA WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN4366164 ELISA IHC IHC (p) WB Rabbit Center Log in to see Polyclonal
1 ABIN1887553 IHC ELISA WB Rabbit Center Log in to see Polyclonal
1 ABIN1585511 IHC ELISA WB Rabbit Log in to see Polyclonal

General

Antigen Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(61), (33), (25), (4), (3), (3), (3), (3), (2), (2), (2), (2)
Host Rabbit
(47), (14), (1)
Clonality
Polyclonal
Conjugate This WNT10B antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(37), (25), (23), (18), (13), (9), (5), (3), (3), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-WNT10B Antibody

Target Details WNT10B Application Details Handling References for anti-WNT10B antibody (ABIN1003488) Images
Blocking Peptide Blocking peptide for this product available: ABIN1004506
Purification Affinity chromatography purified via peptide column.
Immunogen Wnt10b antibody was raised in rabbits against a 15 amino acid peptide from near the center of human Wnt10b.
Isotype IgG

Target Details WNT10B

Product Details anti-WNT10B Antibody Application Details Handling References for anti-WNT10B antibody (ABIN1003488) Images back to top
Antigen
Alternative Name Wnt10b (Wnt12) (WNT10B Antibody Abstract)
Background Wnt-10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt-10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt-10b have also been detected in human breast carcinomas. Wnt-10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt-10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt-10b has multiple roles in cell growth and differentiation. Two isoforms of Wnt-10b are known to exist, this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt-10a.
Pathways WNT Signaling, Skeletal Muscle Fiber Development

Application Details

Product Details anti-WNT10B Antibody Target Details WNT10B Handling References for anti-WNT10B antibody (ABIN1003488) Images back to top
Application Notes Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 – 4 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-WNT10B Antibody Target Details WNT10B Application Details References for anti-WNT10B antibody (ABIN1003488) Images back to top
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months

References for anti-WNT10B antibody (ABIN1003488)

Product Details anti-WNT10B Antibody Target Details WNT10B Application Details Handling Images back to top
Product cited in:

Bennett, Hodge, MacDougald, Schwartz: "Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells." in: Biochemical and biophysical research communications, Vol. 302, Issue 1, pp. 12-6, 2003

Bui, Rankin, Smith, Huguet, Ruben, Strachan, Harris, Lindsay: "A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas." in: Oncogene, Vol. 14, Issue 10, pp. 1249-53, 1997

Lee, Lane, Kuo, Shackleford, Leder: "Insertional mutagenesis identifies a member of the Wnt gene family as a candidate oncogene in the mammary epithelium of int-2/Fgf-3 transgenic mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 6, pp. 2268-72, 1995

Nusse, Varmus: "Wnt genes." in: Cell, Vol. 69, Issue 7, pp. 1073-87, 1992