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anti-Mouse (Murine) WNT16 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT16 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) Antibodies
  • zgc:77293
  • E130309I19Rik
  • wingless-type MMTV integration site family, member 16
  • wingless-related MMTV integration site 16
  • LOC100218349
  • LOC100353090
  • WNT16
  • wnt16
  • Wnt16
Epitope
Internal Region
12
11
8
7
3
2
2
1
1
1
Reactivity
Hamster, Human, Mouse (Murine), Rat (Rattus)
59
19
19
7
4
3
3
3
3
1
Host
Rabbit
58
1
Clonality
Polyclonal
Conjugate
This WNT16 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
35
21
19
13
12
1
1
1
Supplier
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Catalog No. ABIN1049472
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1387899 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN1420810 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN1402939 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal

Similar anti-WNT16 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Hamster
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Western Blotting (WB) 35 Antibodies 7 Antibodies 7 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies 4 Antibodies 4 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies 13 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
ELISA 19 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) Antibodies
Epitope Internal Region
(12), (11), (8), (7), (3), (2), (2), (1), (1), (1)
Reactivity Hamster, Human, Mouse (Murine), Rat (Rattus)
(59), (19), (19), (7), (4), (3), (3), (3), (3), (1)
Host Rabbit
(58), (1)
Clonality
Polyclonal
Conjugate This WNT16 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(35), (21), (19), (13), (12), (1), (1), (1)
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Product Details anti-WNT16 Antibody

Target Details WNT16 Application Details Handling Images
Brand IHC-plus™
Specificity Human WNT16. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CETN3 (50 % ).
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Mouse, Rat, Hamster (100%) Gorilla, Monkey, Marmoset (94%) Gibbon, Elephant, Panda, Dog, Horse, Turkey (88%) Bovine, Rabbit, Opossum, Platypus (81%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human WNT16. Percent identity with other species by BLAST analysis: Human, Mouse, Rat, Hamster (100%), Gorilla, Monkey, Marmoset (94%), Gibbon, Elephant, Panda, Dog, Horse, Turkey (88%), Bovine, Rabbit, Opossum, Platypus (81%).

Type of Immunogen: Synthetic peptide

Target Details WNT16

Product Details anti-WNT16 Antibody Application Details Handling Images back to top
Antigen
Alternative Name WNT16 (WNT16 Antibody Abstract)
Background Name/Gene ID: WNT16
Family: WNT

Synonyms: WNT16, Protein Wnt-16
Gene ID 51384
Pathways WNT Signaling

Application Details

Product Details anti-WNT16 Antibody Target Details WNT16 Handling Images back to top
Application Notes Approved: IHC-P (10 μg/mL)
Comment

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-WNT16 Antibody Target Details WNT16 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Expiry Date 12 months

Images

Product Details anti-WNT16 Antibody Target Details WNT16 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-WNT16 antibody (Wingless-Type MMTV Integration Site Family, Member 16) (Internal Region) (ABIN1049472) Anti-WNT16 antibody ABIN1049472 IHC staining of human kidney. Immunohistochemistry o...