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anti-Mouse (Murine) WNT2B antibody for Immunocytochemistry

Recommended WNT2B Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) Antibodies
  • wnt2b
  • WNT2B
  • Wnt13
  • WNT13
  • XWNT2
  • XWnt-2
  • Xwnt-2b
  • wnt-2b
  • xwnt2b
  • wingless-type MMTV integration site family, member 2Ba
  • wingless-type MMTV integration site family, member 2B
  • wingless related MMTV integration site 2b
  • wnt2ba
  • WNT2B
  • LOC100227493
  • LOC100349847
  • Wnt2b
  • wnt2b-a
Reactivity
Human, Mouse (Murine)
63
34
33
13
12
11
11
10
9
8
7
5
2
1
1
1
Host
Goat
63
1
Clonality
Polyclonal
Conjugate
This WNT2B antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
32
24
14
13
4
2
1
1
1
Supplier
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Catalog No. ABIN293249
$ 969.83
Plus shipping costs $45.00

Similar anti-WNT2B Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 3 Antibodies 4 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 5 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 24 Antibodies
Western Blotting (WB) 15 Antibodies 32 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) Antibodies
Reactivity Human, Mouse (Murine)
(63), (34), (33), (13), (12), (11), (11), (10), (9), (8), (7), (5), (2), (1), (1), (1)
Host Goat
(63), (1)
Clonality
Polyclonal
Conjugate This WNT2B antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
(32), (24), (14), (13), (4), (2), (1), (1), (1)
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Product Details anti-WNT2B Antibody

Target Details WNT2B Application Details Handling Images
Specificity Selected for its ability to recognize mouse and human Wnt-2b in the applications listed.
Purification Affinity purified
Immunogen E. coli-derived rmWnt-2b.
Isotype IgG

Target Details WNT2B

Product Details anti-WNT2B Antibody Application Details Handling Images back to top
Antigen
Alternative Name WNT2B (WNT2B Antibody Abstract)
Background Name/Gene ID: WNT2B
Family: WNT

Synonyms: WNT2B, Protein Wnt-2b, XWNT2, Protein Wnt-13, WNT13, XWNT2, Xenopus, homolog of
Gene ID 7482
UniProt Q93097
Research Area Cancer, Wnt, Wnt Signaling
Pathways WNT Signaling

Application Details

Product Details anti-WNT2B Antibody Target Details WNT2B Handling Images back to top
Application Notes Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Wnt-2b. The detection limit for rmWnt-2b is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows approximately 20 % cross-reactivity with rhWnt-2, and less than 5 % cross-reactivity with rhWnt-7a, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, -16. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect Wnt-2b in BT20 human breast carcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights-557-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Wnt-2b. The detection limit for rmWnt-2b is approximately 0.5 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-WNT2B Antibody Target Details WNT2B Application Details Images back to top
Format Lyophilized
Reconstitution deionized water
Concentration Lot specific
Buffer Lyophilized.