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anti-Human WNT9A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT9A Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) Antibodies
  • wnt-9a
  • WNT-14
  • WNT14
  • Wnt14
  • zgc:153617
Epitope
N-Term
16
16
11
7
6
3
3
3
1
Reactivity
Human
48
10
7
Host
Rabbit
39
8
Clonality
Polyclonal
Conjugate
This WNT9A antibody is un-conjugated
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
30
20
7
6
4
2
1
1
Supplier
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Catalog No. ABIN1049488
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008982679 ABIN1109515 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN1049487 IHC (p) Rabbit Internal Region Log in to see Polyclonal
0.0008982679 ABIN675533 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN1740843 IHC (p) Rabbit IgG Internal Region Log in to see Polyclonal
0.0008982679 ABIN1740844 IHC (p) Rabbit IgG N-Term Log in to see Polyclonal
0.0008982679 ABIN2764567 IHC (p) Rabbit N-Term Log in to see Polyclonal
0.0008982679 ABIN2764568 IHC (p) Rabbit Internal Region Log in to see Polyclonal

Similar anti-WNT9A Antibodies

Application / Reactivity Human
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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
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General

Antigen Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) Antibodies
Epitope N-Term
(16), (16), (11), (7), (6), (3), (3), (3), (1)
Reactivity Human
(48), (10), (7)
Host Rabbit
(39), (8)
Clonality
Polyclonal
Conjugate This WNT9A antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(30), (20), (7), (6), (4), (2), (1), (1)
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Product Details anti-WNT9A Antibody

Target Details WNT9A Application Details Handling Images
Specificity Human WNT9A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Cross-Reactivity Monkey
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%) Mouse, Rat, Bovine, Bat, Rabbit (94%).
Purification Immunoaffinity Chromatography
Immunogen Synthetic 16 amino acid peptide from N-Terminus of human WNT9A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%), Mouse, Rat, Bovine, Bat, Rabbit (94%).
ImmunogenType: Synthetic peptide

Target Details WNT9A

Product Details anti-WNT9A Antibody Application Details Handling Images back to top
Antigen
Alternative Name WNT14 / WNT9A (WNT9A Antibody Abstract)
Background Standard Gene Symbol: WNT9A, Gene Family: WNT, Synonyms: WNT9A, Protein Wnt-14, Protein Wnt-9a, WNT14
Gene ID 7483
Pathways WNT Signaling

Application Details

Product Details anti-WNT9A Antibody Target Details WNT9A Handling Images back to top
Application Notes IHC-P (10 μg/mL),
Comment

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-WNT9A Antibody Target Details WNT9A Application Details Images back to top
Concentration 1 mg/mL
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-80 °C
Storage Comment Long term: -70°C, Short term: +4°C

Images

Product Details anti-WNT9A Antibody Target Details WNT9A Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-WNT9A antibody (Wingless-Type MMTV Integration Site Family, Member 9A) (N-Term) (ABIN1049488) Anti-WNT9A antibody ABIN1049488 IHC staining of human brain, cortex. Immunohistochem...