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anti-Human WNT9B antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT9B Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B) Antibodies
  • clf
  • clf1
  • wnt-9b
  • wnt-14b
  • wnt-15
  • WNT9B
  • WNT14B
  • Wnt14b
  • WNT15
  • Wnt15
Epitope
Internal Region
11
5
2
2
2
1
Reactivity
Human
39
7
4
2
1
1
1
1
1
Host
Rabbit
24
15
2
Clonality
Polyclonal
Conjugate
This WNT9B antibody is un-conjugated
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
30
18
10
10
1
Supplier
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Catalog No. ABIN1049489
€ 429.52
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000896905 ABIN1049490 IHC (p) Rabbit Internal Region Log in to see Polyclonal
0.000896905 ABIN1740846 IHC (p) Rabbit IgG Internal Region Log in to see Polyclonal
0.000896905 ABIN1740845 IHC (p) Rabbit IgG Internal Region Log in to see Polyclonal
0.000896905 ABIN4366213 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
0.000896905 ABIN4366208 IHC (p) Rabbit Log in to see Polyclonal
0.000896905 ABIN4366210 IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.000896905 ABIN4366211 IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.000896905 ABIN4366212 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
0.000896905 ABIN2764587 IHC (p) Rabbit Internal Region Log in to see Polyclonal
0.000896905 ABIN2764588 IHC (p) Rabbit Internal Region Log in to see Polyclonal

Similar anti-WNT9B Antibodies

Application / Reactivity Human
ELISA 16 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Western Blotting (WB) 28 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B) Antibodies
Epitope Internal Region
(11), (5), (2), (2), (2), (1)
Reactivity Human
(39), (7), (4), (2), (1), (1), (1), (1), (1)
Host Rabbit
(24), (15), (2)
Clonality
Polyclonal
Conjugate This WNT9B antibody is un-conjugated
(1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(30), (18), (10), (10), (1)
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Product Details anti-WNT9B Antibody

Target Details WNT9B Application Details Handling Images
Specificity Human WNT9B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Cross-Reactivity Cow (Bovine), Hamster, Horse (Equine), Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig (100%) Bat, Rabbit (94%) Dog (88%).
Purification Immunoaffinity Chromatography
Immunogen Synthetic 16 amino acid peptide from internal region of human WNT9B. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig (100%), Bat, Rabbit (94%), Dog (88%).
ImmunogenType: Synthetic peptide

Target Details WNT9B

Product Details anti-WNT9B Antibody Application Details Handling Images back to top
Antigen
Alternative Name WNT9B / WNT15 (WNT9B Antibody Abstract)
Background Standard Gene Symbol: WNT9B, Gene Family: WNT, Synonyms: WNT9B, WNT14B, WNT15, Protein Wnt-14b, Protein Wnt-15, Protein Wnt-9b
Gene ID 7484
Pathways WNT Signaling

Application Details

Product Details anti-WNT9B Antibody Target Details WNT9B Handling Images back to top
Application Notes IHC-P (10 μg/mL),
Comment

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-WNT9B Antibody Target Details WNT9B Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-80 °C
Storage Comment Long term: -70°C, Short term: +4°C

Images

Product Details anti-WNT9B Antibody Target Details WNT9B Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-WNT9B antibody (Wingless-Type MMTV Integration Site Family, Member 9B) (Internal Region) (ABIN1049489) Anti-WNT9B antibody ABIN1049489 IHC staining of human fetal kidney. Immunohistochemi...