anti-Amiloride-Sensitive Cation Channel 3 (ACCN3) Antibodies

ACCN3 encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Additionally we are shipping Amiloride-Sensitive Cation Channel 3 Proteins (4) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
ACCN3 286920 O35240
ACCN3 9311 Q9UHC3
ACCN3 171209 Q6X1Y6
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Top anti-Amiloride-Sensitive Cation Channel 3 Antibodies at

Showing 10 out of 71 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Human Rabbit Un-conjugated WB Western blot analysis of ASIC3 using anti- ASIC3 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: Rat Brain Tissue Lysate,  Lane 2: Rat Testis Tissue Lysate,  Lane 3: U87 Cell Lysate,  Lane 4: NEURO Cell Lysate,  Lane 5: SMMC Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ASIC3 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC3 at approximately 69KD. The expected band size for ASIC3 is at 58KD. Flow Cytometry analysis of U937 cells using anti-ASIC3 antibody . Overlay histogram showing U937 cells stained with  (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC3 Antibody (,1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. 100 μg 4 to 6 Days
Cow Rabbit Un-conjugated IHC, WB Human kidney WB Suggested Anti-ACCN3 Antibody Titration:  2.5ug/ml  ELISA Titer:  1:62500  Positive Control:  Human Kidney 100 μL 2 to 3 Days
Human Rabbit Un-conjugated ELISA, WB 100 μL Available
Cow Rabbit Un-conjugated IHC, IHC (p), WB 100 μL 11 to 14 Days
Mouse Rabbit Un-conjugated WB Western blot analysis of extract of various cells, using ASIC3 antibody. Western blot analysis of extracts of various cell lines, using ASIC3 antibody (ABIN4902956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. 100 μL 11 to 13 Days
Human Rabbit Un-conjugated WB Western blot analysis in CEM cell line lysates (35ug/lane). 400 μL 2 to 3 Days
Human Rabbit Un-conjugated ELISA, WB 200 μL 12 to 14 Days
Rat Guinea Pig Un-conjugated ICC, IHC   50 μL 11 to 14 Days
Human Rabbit Un-conjugated IHC, WB ACCN3 antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Epithelial cells of renal tubule (arrows) in Human Kidney. Magnification is at 400X ACCN3 antibody used at 2.5 ug/ml to detect target protein. 100 μg 9 to 11 Days
Human Rabbit Un-conjugated IHC, ELISA, WB 100 μL 2 to 3 Days

Top referenced anti-Amiloride-Sensitive Cation Channel 3 Antibodies

  1. Human Polyclonal ACCN3 Primary Antibody for WB - ABIN3042990 : Schuhmacher, Callejo, Srivats, Smith: Naked mole-rat acid-sensing ion channel 3 forms nonfunctional homomers, but functional heteromers. in The Journal of biological chemistry 2018 (PubMed)

More Antibodies against Amiloride-Sensitive Cation Channel 3 Interaction Partners

Horse (Equine) Amiloride-Sensitive Cation Channel 3 (ACCN3) interaction partners

  1. Muscles from horses submitted to strenuous exercises produce lactic acid, which may induce variable pain through ACCN differential properties; ACCN1 and ACCN3 genes have an ubiquitous expression but ACCN1 is more highly expressed in the spinal cord.

Human Amiloride-Sensitive Cation Channel 3 (ACCN3) interaction partners

  1. The present review discusses the dual nature of ASIC3 in joint inflammation with possible mechanisms.

  2. Acidic pH resulted in an increase in ASIC-3 expressionin the discs of paitents with intervertebral disc degeneration.

  3. the data demonstrate a functional link between ASICs and [Ca(2+)]i/RhoA pathway, which contributes to the acidity-induced epithelial-mesenchymal transition.

  4. Respiratory syncytial virus and measles virus infection upregulates ASICS3 expression in bronchial epithelial cells.

  5. The results show that acid-sensing ion channel 1, acid-sensing ion channel 2, and acid-sensing ion channel 3 are expressed in A549 cells at the messenger RNA and protein levels, and acid-sensing ion channel-like currents were elicited by extracellular acid stimuli.

  6. Blocking ASIC3 in interneurons from temporal lobe epilepsy patients decreased the frequency of action potential firing.

  7. These findings open new perspectives on the roles of ASIC3 in the absence of tissue pH variation, as well as on the contribution of those channels to lipid-mediated signaling.

  8. This study suggests that ASIC23may play a role as mediators of inflammatory pain and be involved in the pathogenesis of frozen shoulder.

  9. Sea anemone peptide with uncommon beta-hairpin structure inhibits acid-sensing ion channel 3 (ASIC3) and reveals analgesic activity.

  10. Experiments with Asic3 inhibitors show that Asic3 inhibition leads to loss of pressure-induced vasodilation due to pressure detection failure rather than endothelial mechanisms.

  11. The highly proton sensitive ASIC3 channels are predominantly distributed in peripheral sensory neurons, correlating with their roles in multimodal sensory perception, including nociception, mechanosensation, and chemosensation.

  12. CAR and ASIC3 co-immunoprecipitate only when co-expressed with PSD-95.

  13. Lignan from thyme possesses inhibitory effect on ASIC3 channel current.

  14. A mechanism of induction of ASIC-3 expression relevant to AR was suggested by the finding that eosinophil peroxidase (EPO), acting via ERK1/2, induced the expression of ASIC-3 in epithelial cells.

  15. ASIC3 is able to sense the extracellular pH in both directions and to dynamically adapt its activity between pH 5.0 and 8.0, playing a role in fine tuning neuronal membrane potentials and neuron sensitization in various pH environments.

  16. The results showed that there was a significant increase in the mean relative optical density of ASIC2 and ASIC3 but not ASIC1a in the lining epithelium and glandular tubes of gastric mucosa in patients with Henoch-Schonlein purpura.

  17. Atomic level characterization of the nonproton ligand-sensing domain of ASIC3 channels.

  18. The biological features of ASIC3 is discussed and recent advances on the role of ASIC3 in the pathogenesis and treatment of arthritis pain, are summarized.

  19. Used coincidentally by sensory neurons to detect lactic acidosis, ASIC3 (amiloride-sensitive cation channel 3) interacts with adenosine triphosphate (ATP) to detect muscle ischemia.

  20. Neither ASIC3 or ASIC1 knockout influences the development or maintenance of experimental neuropathic pain after nerve injury.

Mouse (Murine) Amiloride-Sensitive Cation Channel 3 (ACCN3) interaction partners

  1. that ASIC3 plays a role in the control of bladder function by modulating the response of afferents to filling

  2. High ASIC3 expression is associated with Fibromyalgia Pain.

  3. This is the first study to provide evidence that TDAG8, ASIC3 and TRPV1 regulate hyperalgesic priming of RA pain and that a transition from acute to chronic RA pain is at about 4 weeks.

  4. ASICs promote the inflammatory response and apoptosis of RAW 264.7 cells.

  5. Targeted knockout of Asic3 disrupts spindle afferent sensitivity to dynamic stimuli and impairs mechanotransduction in dorsal root ganglion neurons because of substrate deformation-induced neurite stretching, but not to direct neurite indentation.

  6. acid-sensing ion channel 3, activated by concomitant acid and certain pruritogens, constitute a novel signaling pathway that counteracts itch tachyphylaxis to successive pruritogenic stimulation.

  7. a small conopeptide of only four amino acids from the venom of Conus textile that strongly potentiated currents of ASIC3, which has a specific role in the pain pathway. This peptide, RPRFamide, belongs to the subgroup of cono-RFamides.

  8. High ASIC3 expression is associated with Bone Pain Induced by Multiple Myeloma.

  9. This study suggested that the macrophage- and neutrophil-produced IL1beta in the muscles during I/R activates enhanced IL1r1 in muscle afferents increase expression of ASIC3 possibly via JNK activation.

  10. segmental analgesia of electroacupuncture-induced analgesia (EA) with lower intensity is partially mediated by ASIC3 receptor on Abeta-fiber, whereas systemic analgesia induced by EA with higher intensity is more likely induced by TRPV1 receptor on Adelta- and C-fibers

  11. the potentiation of ASICs by quinine depends on the presence of the ASIC1a, ASIC2a subunits, but not ASIC1b, ASIC3 subunits. Furthermore, we have determined the amino acids in ASIC1a that are involved in the modulation of ASICs by pHi.

  12. fatigue reduces pH in muscle that subsequently activates ASIC3 on macrophages to enhance hyperalgesia to muscle insult.

  13. involved in skeletal pain due to high bone turnover

  14. ASIC3 is critical for scratching behavior and pathological changes in chronic itch.

  15. Data suggest that activation of acid-sensing ion channel 3 (ASIC3) on fibroblast-like synoviocytes (FLS) by acidic pH from an inflamed joint could limit synovial proliferation resulting in reduced inflammatory mediators and subsequent joint damage.

  16. we unraveled an important role of ASIC3 in regulating cardiac autonomic function, whereby loss of ASIC3 alters the normal physiological response to ischemic stimuli.

  17. The activation of asic3 activity are essential for the development of long-lasting hyperalgesia in acid-induced, chronic, widespread muscle pain.

  18. Sea anemone peptide with uncommon beta-hairpin structure inhibits acid-sensing ion channel 3 (ASIC3) and reveals analgesic activity.

  19. Local pressure induced vasodilation in Asic3+/+ but not Asic3-/- mice, in which high pressure caused ischemia & ulcers.

  20. Responses of glomus cells to hypoxia and acidosis are uncoupled, reciprocal and linked to ASIC3 expression

Amiloride-Sensitive Cation Channel 3 (ACCN3) Antigen Profile

Protein Summary

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 2 has been observed as proton-gated channels sensitive to gadolinium. Alternatively spliced transcript variants have been described.

Gene names and symbols associated with ACCN3

  • amiloride-sensitive cation channel 3 (ACCN3) antibody
  • acid sensing ion channel subunit 3 (ASIC3) antibody
  • acid sensing ion channel subunit 3 (Asic3) antibody
  • acid-sensing (proton-gated) ion channel 3 (Asic3) antibody
  • Accn3 antibody
  • AW742291 antibody
  • DRASIC antibody
  • SLNAC1 antibody
  • TNAC1 antibody

Protein level used designations for ACCN3

amiloride-sensitive cation channel 3 , amiloride-sensitive cation channel 3-like , DRASIC , acid sensing ion channel 3 , acid-sensing ion channel 3 , dorsal root ASIC , proton gated cation channel DRASIC , amiloride-sensitive cation channel 3, testis , modulatory subunit of ASIC2a , neuronal amiloride-sensitive cation channel 3 , proton-gated cation channel subunit , testis sodium channel 1

429486 Gallus gallus
100063408 Equus caballus
100350503 Oryctolagus cuniculus
100442058 Pongo abelii
286920 Rattus norvegicus
9311 Homo sapiens
171209 Mus musculus
482801 Canis lupus familiaris
789132 Bos taurus
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