anti-Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1) Antibodies

The protein encoded by ID1 is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. Additionally we are shipping ID1 Proteins (8) and ID1 Kits (4) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
ID1 15901  
ID1 25261 P41135
ID1 3397 P41134
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Top anti-ID1 Antibodies at antibodies-online.com

Showing 10 out of 71 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Mouse Un-conjugated ELISA, IF, WB Immunofluorescence of monoclonal antibody to ID1 on HeLa cell (antibody concentration 10 µg/mL). ID1 monoclonal antibody (M04), clone 4G11 Western Blot analysis of ID1 expression in Y-79. 100 μg Log in to see 8 to 11 Days
$527.50
Details
Human Rabbit Un-conjugated FACS, IF, IHC (p), WB Confocal immunofluorescent analysis of ID1 Antibody (Center)(ABIN655663) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). Overlay histogram showing HepG2 cells stained with ABIN655663,ABIN2845135 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (ABIN655663,ABIN2845135, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Dilution: 1:25 400 μL Log in to see 10 to 11 Days
$385.00
Details
Human Rabbit Un-conjugated IHC, WB WB Suggested Anti-ID1 Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:7812500  Positive Control:  Human brain Rabbit Anti-ID1 Antibody   Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated EIA, FACS, IF, IHC (p), WB Flow cytometric analysis of U251 cells using ID1 / BHLHB24 Antibody (Center) Cat.-No AP52144PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Confocal immunofluorescent analysis of ID1 / BHLHB24 Antibody (Center) Cat.-No AP52144PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated WB Western blot analysis of ID1 expression in HeLa (A), HepG2 (B), mouse liver (C), rat liver (D) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated FACS, ICC, IF, IHC, IHC (p), WB Flow Cytometry: ID1 Antibody (JM92-13)  - Analysis of HepG2 cells with Id1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Immunocytochemistry/Immunofluorescence: ID1 Antibody (JM92-13)  - Staining Id1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 100 μL Log in to see 34 Days
$375.73
Details
Human Rabbit Un-conjugated ELISA, ICC, IF, IHC, WB Western blot analysis Id1 using Jurkat whole cell lysates ABIN6274019 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. 100 μL Log in to see 11 to 12 Days
$390.77
Details
Cow Rabbit Un-conjugated IHC, WB Rabbit Anti-ID1 Antibody   Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: N/A Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec Rabbit Anti-ID1 Antibody   Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated FACS, IF, IHC, ELISA, WB Confocal immunofluorescent analysis of ID1 antibody with U-251MG cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue). ID1 antibody flow cytometric analysis of U251 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis. 0.4 mL Log in to see 2 to 3 Days
$452.38
Details
Human Mouse Un-conjugated IF, ELISA, WB Detection limit for recombinant GST tagged ID1 is approximately 1ng/ml as a capture antibody. Western Blot detection against Immunogen (38.1 KDa) . 100 μg Log in to see 11 to 12 Days
$440.00
Details

Top referenced anti-ID1 Antibodies

  1. Human Monoclonal ID1 Primary Antibody for ELISA, WB - ABIN561409 : Chen, Forootan, Gosney, Forootan, Ke: Increased expression of Id1 and Id3 promotes tumorigenicity by enhancing angiogenesis and suppressing apoptosis in small cell lung cancer. in Genes & cancer 2014 (PubMed)

  2. Human Monoclonal ID1 Primary Antibody for IF, ELISA - ABIN561410 : Phi, Choi, Lim, Lee, Wang, Park, Kim: ID3 contributes to cerebrospinal fluid seeding and poor prognosis in medulloblastoma. in BMC cancer 2013 (PubMed)

More Antibodies against ID1 Interaction Partners

Zebrafish Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1) interaction partners

  1. Gain and loss of id1 function in vivo demonstrated that Id1 promotes stem cell quiescence. The increased id1 expression observed in neural stem cells in response to injury appeared independent of inflammatory signals.

Mouse (Murine) Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1) interaction partners

  1. We show that expression of Id-1, a negative regulator of myogenesis, is enhanced in Dnmt1-deficient cultures, leading to enhanced transdifferentiation of myoblasts toward the osteogenic lineage. Thus, these studies demonstrate that Dnmt1 influences cellular identity and determines lineage fidelity.

  2. a loss of CULLIN3 represents a common signaling node for controlling the activity of intracellular WNT and SHH signaling pathways mediated by ID1

  3. These results reveal a E2F1/miR-17-92/ENH1/Id1 regulatory axis during myogenesis.

  4. Id1 enables both Lewis lung carcinoma cells and the host microenvironment for an effective liver colonization.

  5. adipose tissue-specific overexpression of Id1 causes age-associated and high-fat diet-induced obesity in mice. Id1 suppresses BAT thermogenesis by binding to and suppressing PGC1alpha transcriptional activity. Lack of Id1 increases beige gene and Ucp1 expression in the WAT in response to cold exposure. Furthermore, brown-like differentiation is increased in Id1-deficient mouse embryonic fibroblasts.

  6. If Gfi1 levels fall below a threshold, Id1 expression increases and renders E2A unable to function, which prevents hematopoietic progenitors from engaging along the B lymphoid lineage

  7. Id1 may promote cell cycle progression of endothelial progenitor cells (EPCs), and that Wnt2 may be important in Id1 regulation of the cell cycle of EPCs.

  8. Differential role of Id1 in MLL-AF9-driven leukemia based on cell of origin

  9. Collectively, our results suggest that ENH1 plays an important role in the activation of myogenesis through the repression of Id2 activity.

  10. Data indicate that upregulation of E2-2 protein markedly attenuated the inhibitor of DNA-binding 1 (ID1)-mediated increase in endothelial progenitor cells (EPCs) proliferation and migration.

  11. these findings demonstrate that loss of Id compensation leads to dysregulation of the hematopoietic transcriptional network and multiple defects in erythropoietic development in adult mice.

  12. our results strongly suggest that Usp1 is involved in the regulation of centrosome duplication, at least in part via ID1, and Usp1 may exert its oncogenic activity, partially through inducing centrosome abnormality.

  13. demonstrated that Id1 and E2-2 are critical regulators of EPCs function in vitro. Id1 interacts with E2-2 and relieves the E2-2-mediated repression of FGFR1 and VEGFR2 expression to modulate EPCs functions

  14. Endothelial ID1 up-regulation in inflamed colonic mucosa is an adaptive response that modulates the severity of tissue injury.

  15. these findings provide in vivo genetic evidence of Id1 functions as an oncogene in breast cancer

  16. overexpression leads to systemic immunosuppression by downregulation of key molecules involved in dendritic cell differentiation and suppression of CD8 T-cell proliferation, thus promoting primary tumour growth and metastatic progression

  17. ID1/3 are crucial regulators of the adaptive antioxidant-mitochondrial response that promotes beta cell survival during oxidative stress through a novel link to the NFE2L2-small MAF pathway.

  18. Using genetically modified mice, we found that loss of Id1 inhibited t(8;21) leukemia initiation and progression in vivo by abrogating protein kinase B (AKT)1 activation, and that Id1 interacted with AKT1 through its C terminus.

  19. We conclude that Id1 is potently angiogenic and can be up-regulated in endothelial progenitor cells

  20. Direct interaction between Id1 and Zrf1 blocks Zrf1 recruitment to chromatin thus impairing neural commitment of embryonic stem cells.During embryonic stem cell differentiation, Id1 levels decrease allowing Zrf1 to activate neural genes.

Human Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1) interaction partners

  1. Low ID1 expression is associated with breast cancer.

  2. ID1, CTCF and ELK1 may be associated with prostate cancer, and may be potential therapeutic targets for the treatment of this disease.

  3. CaMKII can directly phosphorylate Beclin 1 at Ser90 to promote K63-linked ubiquitination of Beclin 1 and activation of autophagy; it also promotes K63-linked ubiquitination of inhibitor of differentiation 1/2 (Id-1/2) by catalyzing phosphorylation of Id proteins and recruiting TRAF-6

  4. ID1 expression was associated with the proliferation, invasion and migration of SACC cells. The observed inhibition of SACC cell growth, invasion and migration following knockdown of ID1 expression in the present study, may have been due to restoration of the balance between oncogenic and tumor-suppressive effects resulting from changes in the expression of downstream genes or associated proteins.

  5. The mutual correlation between the expression level of TWIST1 and ID1 might be a critical factor driving the process of the human odontoblasts' differentiation.

  6. a loss of CULLIN3 represents a common signaling node for controlling the activity of intracellular WNT and SHH signaling pathways mediated by ID1

  7. Id1 enables lung cancer liver colonization by activating an epithelial mesenchymal transformation program in tumor cells and establishing the pre-metastatic niche.

  8. High ID1 expression is associated with breast cancer.

  9. ID1 expression impacts the sensitivity of colon cancer cells to 5-FU and may be considered as a potential predictive marker in colorectal carcinoma treatment.

  10. Reducing ID1 gene expression reduces metastatic spread of salivary gland neoplasms.

  11. High ID-1 expression is associated with colorectal carcinoma.

  12. ID1 expression was positively related to drug resistance of EGFR-TKI in non-small cell lung cancer.

  13. MVD was determined by immunohistochemistry, and the expressions of mRNA and protein of inhibitor of differentiation-1 (ID1) and vascular endothelial growth factor (VEGF) were detected in gastric cancer.

  14. ID1 down-regulation induced parallel changes in the IGF and AKT pathways. The crosstalk of these pathways may enhance malignant phenotypes in salivary gland cancer.

  15. Results show that ID1, ID3 and IGJ genes are highly expressed in adult B-ALL and correlate with poor prognosis in Hispanic patients.

  16. Our findings define an intricate E2F1-dependent mechanism by which Id1 increases thymidylate synthase and IGF2 expressions to promote cancer chemoresistance. The Id1-E2F1-IGF2 regulatory axis has important implications for cancer prognosis and treatment.

  17. Peritoneal VEGF-A expression is regulated by TGF-beta1 through an ID1 pathway in women with endometriosis

  18. Id-1, a protein repressed by miR-29b, facilitates TGFbeta1-induced EMT in human ovarian cancer cells and represents a promising therapeutic target for treating ovarian cancer.

  19. Simultaneous high expression of ID1 and c-Jun or c-Fos was correlated with poor survival in esophageal squamous cell carcinoma patients.

  20. Id1 as an important modulator of molecular events during DPSC commitment and differentiation, which should be considered in dental research on tissue engineering.

ID1 Antigen Profile

Protein Summary

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with ID1

  • inhibitor of DNA binding 1 (id1) antibody
  • inhibitor of DNA binding 1, HLH protein (ID1) antibody
  • inhibitor of DNA binding 1 (Id1) antibody
  • inhibitor of DNA binding 1, HLH protein (Id1) antibody
  • AI323524 antibody
  • bHLHb24 antibody
  • cb108 antibody
  • D2Wsu140e antibody
  • ID antibody
  • ID1 antibody
  • id6 antibody
  • ID125A antibody
  • Idb1 antibody
  • wu:fb57e11 antibody

Protein level used designations for ID1

inhibitor of DNA binding 1 , DNA-binding protein inhibitor id-1 , inhibitor of DNA binding 1, dominant negative helix-loop-helix protein , DNA-binding protein inhibitor ID-1 , inhibitor of DNA binding 6 , Inhibitor of DNA binding 1 helix-loop-helix protein (splice varaiation) , Inhibitor of DNA binding 1, helix-loop-helix protein (splice variation) , class B basic helix-loop-helix protein 24 , dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) , inhibitor of differentiation 1 , helix-loop-helix protein

GENE ID SPECIES
100528658 Ictalurus punctatus
469912 Pan troglodytes
30493 Danio rerio
15901 Mus musculus
25261 Rattus norvegicus
3397 Homo sapiens
609779 Canis lupus familiaris
100522839 Sus scrofa
497011 Bos taurus
395282 Gallus gallus
Selected quality suppliers for anti-ID1 (ID1) Antibodies
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