anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibodies

displays differential expression after induction by nerve growth factor [RGD, Feb 2006].. Additionally we are shipping NEDD8 Proteins (29) and NEDD8 Kits (6) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
NEDD8 4738 Q15843
NEDD8 18002 P29595
NEDD8 25490 Q71UE8
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Top anti-NEDD8 Antibodies at antibodies-online.com

Showing 10 out of 132 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Yeast Rabbit Un-conjugated EIA, WB Immunoblot of Rub1 fusion protein. Anti-Rub1 antibody, generated by immunization with full-length recombinant yeast Rub1, was tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # 611-703-127) for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche). Other detection systems will yield similar results. 0.5 mg Log in to see 6 to 8 Days
$610.50
Details
Human Rabbit Un-conjugated IHC (p), WB NEDD8 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022). Western blot analysis of NEDD8 expression in rat testis extract ( Lane 1), mouse thymus extract ( Lane 2), mouse brain extract ( Lane 3), HELA whole cell lysates ( Lane 4) and MCF-7 whole cell lysates ( Lane 5). NEDD8 at 9KD was detected using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody (Catalog #) at 0.5 µg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of NEDD8 polyclonal antibody (ABIN388037) in HeLa cell lysate. NEDD8 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma 400 μL Log in to see 10 to 11 Days
$335.50
Details
Cow Rabbit Un-conjugated IP, IHC, WB Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Western blot analysis of NEDD8 expression in BJAB (A), K562 (B), HeLa (C) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Mouse Un-conjugated ELISA, FACS, ICC, IHC, WB Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng); Dilution: 1/10000 Figure 1: Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa) Dilution: 1/500 - 1/2000 100 μL Log in to see 8 to 11 Days
$561.00
Details
Human Rabbit Un-conjugated ELISA, ICC, IF, IHC, WB Western blot analysis on Jurkat cell lysate using NEDD8 Antibody,The lane on the left is treated with the antigen-specific peptide. Western blot analysis of extracts from 293, using NEDD8 Antibody. 100 μL Log in to see 11 to 12 Days
$390.77
Details
Human Mouse Un-conjugated ELISA, FACS, IF, IHC, WB 100 μg Log in to see 7 to 9 Days
$551.83
Details
Human Rabbit Un-conjugated IHC, ELISA, WB Western blot analysis of extracts from Jurkat cells, using NEDD8 Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Mouse Un-conjugated CyTOF, ELISA, FACS, ICC, IF, IHC, WB Flow Cytometry: NEDD8 Antibody (1A7) [NBP2-37524] - Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red). Western Blot: NEDD8 Antibody (1A7) [NBP2-37524] - Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa) 0.1 mL Log in to see 7 to 9 Days
$514.63
Details

Top referenced anti-NEDD8 Antibodies

  1. Human Monoclonal NEDD8 Primary Antibody for ICC, FACS - ABIN969547 : Ohki, Funatsu, Konishi, Chiba: The mechanism of poly-NEDD8 chain formation in vitro. in Biochemical and biophysical research communications 2009 (PubMed)

More Antibodies against NEDD8 Interaction Partners

Human Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) interaction partners

  1. Nedd8 binding to Smurf plays important roles in the regulation of cell migration and the BMP and TGFbeta (show TGFB1 Antibodies) signaling pathways.

  2. NEDD8-mediated neddylation promotes stress granule assembly.

  3. NEDD8 seems to inhibit the Src (show SRC Antibodies)-mediated phosphorylation of caveolin-1 (show CAV1 Antibodies) by modifying the structure of caveolin-1 (show CAV1 Antibodies) protein, which blocks the migration of cancer cells. Although the neddylation process is currently regarded as an emerging target for cancer therapy, our results suggest the possibility that the inhibition of neddylation could facilitate cancer invasion or metastasis at least in some types of cancers.

  4. The cellular effects of NEDD8 inhibition can be manipulated based on the p53 (show TP53 Antibodies) status.

  5. Here we report that high expression of neddylation components, NEDD8 and NAE1 (show NAE1 Antibodies), are associated with poor survival of Pancreatic ductal adenocarcinoma patients. Blockage of neddylation by MLN4924 significantly sensitizes pancreatic cancer cells to gemcitabine, reduced clonogenic survival, decreased invasion capacity, increased apoptosis and senescence

  6. Downregulation of NEDD8 enhanced the susceptibility of nasopharyngeal carcinoma cells to cisplatin and radiation.

  7. Furthermore, the authors characterized SENP8/DEN1 (show SENP8 Antibodies) as the protease that counteracts Ubc12 (show ube2m Antibodies) auto-neddylation, and observed aberrant neddylation of Ubc12 (show ube2m Antibodies) and other NEDD8 conjugation pathway components in SENP8 (show SENP8 Antibodies)-deficient cells.

  8. MKK7 (show MAP2K7 Antibodies) undergoes neddylation in human breast cancer cells

  9. complete blocking of CRLs at a higher inhibitor dose-induced cytotoxicity that was amplified by knockdown of CRL (show IL31RA Antibodies) regulator Cand1 (show CAND1 Antibodies).

  10. In conclusion, the authors revealed that E3 ligase HDM2 promotes NEDD8 NEDDylation of HBx to enhance HBx stability and chromatin localization, which in turn favors HBx-dependent transcriptional regulation, cell proliferation, and hepatitis B virus-driven tumor growth.

Mouse (Murine) Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) interaction partners

  1. Downregulation of NEDD8 enhanced the susceptibility of nasopharyngeal carcinoma cells to cisplatin and radiation.

  2. Data show that NEDL2 regulates GDNF/Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase activity.

  3. neural precursor cell expressed developmentally downregulated protein 8 (Nedd8) modification of Cul-5 (show CUL5 Antibodies) is required for its interaction with TRAF6 (show TRAF6 Antibodies).

  4. Our findings provide mechanistic evidence of the effects of IAP (show ALPI Antibodies) antagonists in combination with NAE inhibitors, and demonstrate the potential of a new combination therapy for cancer.

  5. a role of Nedd8 in regulating caspase-1 (show CASP1 Antibodies) activation following inflammasome activation

  6. Nedd8 and its associated E2 carrier protein (Ubc12 (show ube2m Antibodies)) and E3-SCF (show KITLG Antibodies) ligase play a key role in regulating the expression and activity of ENaC (show SCNN1A Antibodies) in alveolar pneumocytes.

  7. a new regulatory mechanism of RCAN1 (show RCAN1 Antibodies) function

  8. c-Cbl conjugates neural precursor cell-expressed, developmentally downregulated 8 (NEDD8), a ubiquitin-like protein, to TbetaRII at Lys556 and Lys567.

  9. The NEDD8 pathway appears to play an important role in CD4 (show CD4 Antibodies)+ T-cell-mediated inflammatory responses

  10. Nedd8 and AhR (show AHR Antibodies) localize to overlapping areas in the heart and spinal ganglia, raising the possibility that this interaction may play a role in organogenesis

Fruit Fly (Drosophila melanogaster) Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) interaction partners

  1. the Cul1 (show CUL1 Antibodies)-based SCF (show KITLG Antibodies) complex requires Nedd8 modification for the degradation processes of Ci, Arm, and CycE (show CCNE1 Antibodies) in vivo

  2. Genetic analyses indicate that a balance between neddylation and deneddylation maintained by DEN1 (show SENP8 Antibodies) is crucial for animal viability.

NEDD8 Antigen Profile

Protein Summary

displays differential expression after induction by nerve growth factor

Gene names and symbols associated with NEDD8

  • neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) antibody
  • neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8) antibody
  • neural precursor cell expressed, developmentally down-regulated 8 (Nedd8) antibody
  • CG10679 gene product from transcript CG10679-RB (Nedd8) antibody
  • neural precursor cell expressed, developmentally down-regulated 8 (nedd8) antibody
  • Nedd8 (Nedd8) antibody
  • ubiquitin-like protein Nedd8 (nedd8) antibody
  • neural precursor cell expressed, developmentally down-regulated 8 L homeolog (nedd8.L) antibody
  • CG10679 antibody
  • DDBDRAFT_0218116 antibody
  • DDBDRAFT_0238041 antibody
  • DDB_0218116 antibody
  • DDB_0238041 antibody
  • Dmel\\CG10679 antibody
  • dNEDD8 antibody
  • GB17785 antibody
  • NEDD-8 antibody
  • nedd8 antibody
  • nedd8.S antibody
  • Rub1 antibody
  • si:zc14a17.5 antibody
  • ubiquitin antibody

Protein level used designations for NEDD8

neddylin , ubiquitin-like protein Nedd8 , NEDD-8 , NEDD8 , neural precursor cell expressed developmentally down-regulated protein 8 , neural precursor cell expressed, developmentally down-regulated gene 8 , CG10679-PA , Nedd8-PA , neural precursor cell-expressed developmentally downregulated , neural precursor cell expressed, developmentally down-regulated 8 , Nedd8 , NEDD8 protein , neural precursor cell expressed, developmentally down-regulated 8 S homeolog

GENE ID SPECIES
4738 Homo sapiens
18002 Mus musculus
25490 Rattus norvegicus
35151 Drosophila melanogaster
286796 Bos taurus
368667 Danio rerio
480265 Canis lupus familiaris
549727 Xenopus (Silurana) tropicalis
552822 Apis mellifera
715763 Macaca mulatta
8621655 Dictyostelium discoideum AX4
100009008 Oryctolagus cuniculus
100301786 Oncorhynchus mykiss
100528101 Ictalurus punctatus
100520086 Sus scrofa
446966 Xenopus laevis
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