Biotin Mouse Lineage Panel

Catalog No. ABIN2690963

Quick Overview for Biotin Mouse Lineage Panel (ABIN2690963)

Application: Flow Cytometry (FACS)

Product Details

Reactivity: Mouse

Conjugate: Biotin

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD Pharmingen™ Biotin Mouse Lineage Panel - Reactivity Ms

Sample Volume: 2 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. 559971 Rev. 2 Page 1 of 3 Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with BD™ Mouse Lineage Panel and BD™ IMag Streptavidin Particles Plus - MSC (Cat. no. 557811, at 10 μl per 1 x 10^7 total cells). The labeled cells were then separated over a magnetic separation column designed for depletions. To demonstrate the efficiency of the depletion, unmanipulated bone-marrow cells and the lineage-negative fraction were stained with APC-conjugated anti-mouse CD117 mAb 2B8 (Cat. No. 553356) to detect hematopoietic progenitors, and with PE-conjugated anti-mouse TER-119/Erythroid Cells (Cat. No. 553673), PE-conjugated anti-mouse CD45R/B220 (Cat. No. 553089/553090), and PE-conjugated anti-mouse CD11b (Cat. No. 557397/553311) to detect lineage-committed cells. The lineage-negative fraction contains a greatly increased proportion of CD117+ cells and less than 5% of lineage-positive contaminants.

References

Sato, Laver, Ogawa: "Reversible expression of CD34 by murine hematopoietic stem cells." in: Blood, Vol. 94, Issue 8, pp. 2548-54, (1999) (PubMed).

Goodell, Rosenzweig, Kim, Marks, DeMaria, Paradis, Grupp, Sieff, Mulligan, Johnson: "Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species." in: Nature medicine, Vol. 3, Issue 12, pp. 1337-45, (1998) (PubMed).

Morrison, Wandycz, Hemmati, Wright, Weissman: "Identification of a lineage of multipotent hematopoietic progenitors." in: Development (Cambridge, England), Vol. 124, Issue 10, pp. 1929-39, (1997) (PubMed).

Spangrude, Scollay: "A simplified method for enrichment of mouse hematopoietic stem cells." in: Experimental hematology, Vol. 18, Issue 8, pp. 920-6, (1990) (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, (1988) (PubMed).