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CUT&RUN Concanavalin A Beads

Catalog No. ABIN6923139
  • Application
    Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Separation (Sep)
    Purpose
    CUT&RUN Concanavalin A Beads of our CUT&RUN Sets.
    Specificity
    The unique saccharide-binding properties of plant lectins, such as Concanavalin A have made them useful for the labeling and isolation of glycan-presenting cells and glycoproteins in serum and cell lysate.
    Characteristics
    Concanavalin A is covalently attached to functionalized Magnetic iron oxide particles for use in glycoprotein isolations from serum and cellular extracts.
    Components
    Concanavalin A coated particles
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    • Metal ions (calcium and manganese) mediate the binding to Concanavalin A and stabilize is conformation.
    • The use of buffers with EDTA or other metal chelators must be avoided as it will result in a loss of carbohydrate binding ability.

    Reagent Preparation

    Prepare CUT&RUN Concanavalin A Beads

    • Pipette 10 µL CUT&RUN Concanavalin A Beads slurry for each sample into a tube
    • Place the tubes on a magnet separator and remove the liquid carefully
    • Remove the tubes from the magnetic separator
    • Wash beads 3 more times with 1 mL Binding Buffer
    • Finally resuspend the beads with 10 µL Binding Buffer per sample
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    5 μg/μL
    Buffer
    Stored in 10 mM PBS with 0.1 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze the product!
    Vortex bead suspension well before use.
    Storage
    4 °C
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