Rhinovirus 16 antibody

Catalog No. ABIN1000236

Product Details

Target: Rhinovirus 16

Reactivity: Human Rhinovirus (HRV)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: Anti-Rhinovirus 16 Mouse Monoclonal Antibody

Specificity: This non-neutralizing antibody recognizes capsid protein VP2 (mw ~30 kDa) of HRV16, HRV1A, and HRV39 and VP2 precursors VP0 (mw ~37 kDa) and P1 (mw ~90 kDa).

Cross-Reactivity: Human Rhinovirus (HRV)

Immunogen: Purified HRV16.

Clone: R16-7

Isotype: IgG2b

Application Details

Application Notes: Immunoblotting: use at 2-5 μg/mL.
Immunohistochemistry: use at 1-10 μg/mL.
These are recommended concentrations,
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

References

Angel-Ambrocio, Bautista-Carbajal, García-León, Gomora-Herrera, Pedernera-Astegiano, Wong-Chew: "Microglia HMC3 cells are highly susceptible to Rhinovirus infection." in: Virus research, Vol. 288, pp. 198110, (2020) (PubMed).

Roth, Fang, Stolz, Tamm: "Pelargonium sidoides radix extract EPs 7630 reduces rhinovirus infection through modulation of viral binding proteins on human bronchial epithelial cells." in: PLoS ONE, Vol. 14, Issue 2, pp. e0210702, (2019) (PubMed).

Roth, Pasquali, Stolz, Tamm: "Broncho Vaxom (OM-85) modulates rhinovirus docking proteins on human airway epithelial cells via Erk1/2 mitogen activated protein kinase and cAMP." in: PLoS ONE, Vol. 12, Issue 11, pp. e0188010, (2017) (PubMed).

Wood, Bledsoe, Chai, Daka, Deng, Ding, Harris-Gurley, Kryn, Nartey, Nichols, Nolte, Prabhu, Rise, Sheahan, Shotwell, Smith, Tai, Taylor, Tomberlin, Wang, Wisely, You, Xia, Dickson: "The Role of Phosphodiesterase 12 (PDE12) as a Negative Regulator of the Innate Immune Response and the Discovery of Antiviral Inhibitors." in: The Journal of biological chemistry, Vol. 290, Issue 32, pp. 19681-96, (2015) (PubMed).

Target Details

Target: Rhinovirus 16

Target Type: Virus

Background: Picornaviruses are small, non-enveloped RNA viruses with an icosahedral capsid and a single strand, plus-sense RNA genome. The genome encodes a single polyprotein that is proteolytically processed by viral proteases into structural and non-structural proteins. The family of picornaviruses includes numerous human and animal viruses including more than 100 s of human rhinoviruses (HRV). HRV infections are characteristic upper airway infections (the main cause of the common cold), and they provoke significant lower airway symptoms for patients with asthma, cystic fibrosis, or chronic obstructive pulmonary disease.