GFP antibody (Green Fluorescent Protein) (AA 246) (HRP)

Details for Product anti-GFP Antibody No. ABIN100089
Antigen
Epitope
AA 246
19
16
9
7
5
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2
2
2
2
1
1
1
1
Reactivity
Aequorea victoria
399
10
10
9
9
7
6
3
2
1
Host
Goat
168
155
57
29
16
7
3
2
Clonality
Polyclonal
Conjugate
This GFP antibody is conjugated to HRP
19
15
12
9
6
4
3
3
3
3
3
3
3
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2
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2
2
2
2
2
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2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
342
195
108
87
83
54
27
25
22
20
15
12
10
8
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1
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Options
Immunogen The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Alternative Name GFP
Background HRP Anti-GFP is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: HRP, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
Application Notes Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide!
Storage 4 °C
Supplier Images
Image no. 1 for anti-Green Fluorescent Protein (GFP) (AA 246) antibody (HRP) (ABIN100089) Western Blot of Anti-GFP Antibody Peroxidase Conjugate. Lane 1: 50ng of GFP. Lane 2: ...
Image no. 2 for anti-Green Fluorescent Protein (GFP) (AA 246) antibody (HRP) (ABIN100089) Western Blot of Goat anti-GFP antibody Peroxidase conjugated. Marker: Opal Pre-staine...
Product cited in: Bisgrove, Su, Yost: "Maternal Gdf3 is an obligatory cofactor in Nodal signaling for embryonic axis formation in zebrafish." in: eLife, Vol. 6, 2018 (PubMed).

Botto, Totonchy, Gustin, Moses: "Kaposi Sarcoma Herpesvirus Induces HO-1 during De Novo Infection of Endothelial Cells via Viral miRNA-Dependent and -Independent Mechanisms." in: mBio, Vol. 6, Issue 3, pp. e00668, 2016 (PubMed).

Fahrenkamp, de Leur, Küster, Chatain, Müller-Newen: "Src family kinases interfere with dimerization of STAT5A through a phosphotyrosine-SH2 domain interaction." in: Cell communication and signaling : CCS, Vol. 13, pp. 10, 2015 (PubMed).

Gustin, Bai, Moses, Douglas: "Ebola Virus Glycoprotein Promotes Enhanced Viral Egress by Preventing Ebola VP40 From Associating With the Host Restriction Factor BST2/Tetherin." in: The Journal of infectious diseases, Vol. 212 Suppl 2, pp. S181-90, 2015 (PubMed).

Bejarano, Yuste, Patel, Stout, Spray, Cuervo: "Connexins modulate autophagosome biogenesis." in: Nature cell biology, Vol. 16, Issue 5, pp. 401-14, 2014 (PubMed).

Sung, Lim, Yi, Chang, Yang, Lee, Huh: "Genome-wide bimolecular fluorescence complementation analysis of SUMO interactome in yeast." in: Genome research, Vol. 23, Issue 4, pp. 736-46, 2013 (PubMed).

Chatain, Ziegler, Fahrenkamp, Jost, Moriggl, Schmitz-Van de Leur, Müller-Newen: "Src family kinases mediate cytoplasmic retention of activated STAT5 in BCR-ABL-positive cells." in: Oncogene, 2012 (PubMed).

Ha, Sung, Huh: "Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae." in: Nucleic acids research, Vol. 40, Issue 11, pp. 4892-903, 2012 (PubMed).

Guarino, Shepherd, Salguero, Hua, Deegan, Kearsey: "Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation." in: Nucleic acids research, Vol. 39, Issue 14, pp. 5978-90, 2011 (PubMed).