HA-Tag antibody (Biotin)

Details for Product No. ABIN100177
Antigen
Reactivity
Tag
341
6
6
5
2
1
1
Host
Rabbit
180
179
24
16
3
1
Clonality
Polyclonal
Conjugate
Biotin
31
23
15
8
6
5
5
5
5
5
5
5
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunomicroscopy (IM), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
323
155
105
61
44
30
26
15
13
9
8
7
6
6
5
5
4
4
4
3
3
2
2
2
1
1
1
1
1
Options
Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Sequence YPYDVPDYA
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Target Type Tag
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Application Notes Anti-HA is optimally suited for monitoring the expression of HA tagged fusion proteins.  As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope.  The antibody recognizes the HA epitope tag fused to the amino- terminus of targeted proteins as is expressed in many commonly used expression vectors.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques.  Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Image no. 1 for anti-HA-Tag antibody (Biotin) (ABIN100177) Anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by wes...