HIF3A antibody (Hypoxia Inducible Factor 3, alpha Subunit) (AA 581-592)

Details for Product anti-HIF3A Antibody No. ABIN100475
Antigen
Epitope
AA 581-592
12
11
8
8
6
6
4
3
2
2
2
2
1
Reactivity
Mouse (Murine)
70
44
34
1
Host
Rabbit
59
12
7
Clonality
Polyclonal
Conjugate
This HIF3A antibody is un-conjugated
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
52
26
22
11
9
9
4
1
1
1
Options
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 581-592 of mouse Hif3 a (Hypoxia Inducible Factor).
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Antigen
Alternative Name HIF3 alpha (HIF3A Antibody Abstract)
Background One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three PRIVATE ''TYPE=PICT;ALT={alpha}''subunits (HIF1-aPRIVATE ''TYPE=PICT;ALT={alpha}'', HIF2-a,PRIVATE ''TYPE=PICT;ALT={alpha}'' or HIF3-a)PRIVATE ''TYPE=PICT;ALT={alpha}'' bound to the aryl hydrocarbon receptor nuclear translocator (ARNT) that is also known as HIF1-b. HIF-aPRIVATE ''TYPE=PICT;ALT={alpha}'' is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and angiogenesis . Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive
Synonyms: HIF3 alpha antibody, HIF3A antibody, Hypoxia Inducible Factor 3 alpha antibody, Hypoxia inducible factor 3 alpha subunit antibody, Hypoxia inducible factor three alpha antibody, Inhibitory PAS domain protein antibody, IPAS antibody
Gene ID 53417, 170295859
UniProt Q0VBL6
Application Notes This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 a protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 a due to sequence homology.
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 581-592) antibody (ABIN100475) Western blot using Affinity Purified anti-Hif antibody shows detection of a band ~72...
Product cited in: Takeda, Ho, Takeda, Duan, Nagy, Fong: "Placental but not heart defects are associated with elevated hypoxia-inducible factor alpha levels in mice lacking prolyl hydroxylase domain protein 2." in: Molecular and cellular biology, Vol. 26, Issue 22, pp. 8336-46, 2006 (PubMed).