CD73 antibody

Catalog No. ABIN1027668

This anti-CD73 antibody is a Mouse Monoclonal antibody detecting CD73 in FACS, IHC (fro) and MS. Suitable for Human and Non-Human Primate. This Primary Antibody has been cited in 7+ publications.

Quick Overview for CD73 antibody (ABIN1027668)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Mass Spectrometry (MS)

Clone: AD2

Product Details anti-CD73 Antibody

Purpose: Anti-Hu CD73 Purified

Specificity: The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: pre-B leukemia cells

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD73 antibody (ABIN1027668)

Semenov, Koestenbauer, Riegel, Zech, Zimmermann, Zisch, Malek: "Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation." in: American journal of obstetrics and gynecology, Vol. 202, Issue 2, pp. 193.e1-193.e13, (2010) (PubMed).

Mokry, Soukup, Micuda, Karbanova, Visek, Brcakova, Suchanek, Bouchal, Vokurkova, Ivancakova: "Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells." in: Journal of biomedicine & biotechnology, Vol. 2010, pp. 673513, (2010) (PubMed).

Paquet-Fifield, Schlüter, Li, Aitken, Gangatirkar, Blashki, Koelmeyer, Pouliot, Palatsides, Ellis, Brouard, Zannettino, Saunders, Thompson, Li, Kaur: "A role for pericytes as microenvironmental regulators of human skin tissue regeneration." in: The Journal of clinical investigation, Vol. 119, Issue 9, pp. 2795-806, (2009) (PubMed).

Rodriguez, Paquet, Yang, Erle: "Differential gene expression by integrin beta 7+ and beta 7- memory T helper cells." in: BMC immunology, Vol. 5, pp. 13, (2004) (PubMed).

Hashikawa, Takedachi, Terakura, Saho, Yamada, Thompson, Shimabukuro, Murakami: "Involvement of CD73 (ecto-5'-nucleotidase) in adenosine generation by human gingival fibroblasts." in: Journal of dental research, Vol. 82, Issue 11, pp. 888-92, (2003) (PubMed).

Nemoto, Tada, Shimauchi: "Disruption of CD40/CD40 ligand interaction with cleavage of CD40 on human gingival fibroblasts by human leukocyte elastase resulting in down-regulation of chemokine production." in: Journal of leukocyte biology, Vol. 72, Issue 3, pp. 538-45, (2002) (PubMed).

Borrione, Peola, Mariani, Besostri, Mallone, Malavasi, Pileri, Massaia: "CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 6238-46, (1999) (PubMed).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background: 5'-nucleotidase ecto,CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.,ecto-5´nucleotidase, NT5E, E5NT, CALJA, NTE

Gene ID: 4907

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process