KB-RAS (AA 179-192) antibody

Catalog No. ABIN103357

Supplier Product No.: 600-401-273

This Rabbit Polyclonal antibody specifically detects KB-RAS in ELISA and WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for KB-RAS (AA 179-192) antibody (ABIN103357)

Target: KB-RAS

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Binding Specificity: AA 179-192

Supplier Product No.: 600-401-273

Supplier: Rockland

Purpose: KB-RAS Antibody

Characteristics: Synonyms: rabbit anti-KB-RAS antibody, rabbit anti-Kappa B Ras antibody, I kappa B interacting Ras like protein 1 antibody, Kappa B Ras 1 antibody, Kappa B Ras protein 1 antibody, KappaB Ras1 antibody, KBRAS 1 antibody, KBRAS1 antibody, NF-kappa B inhibitor interacting Ras-like protein 1 antibody

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 179-192 of human KB-RAS. A single amino acid changes of S190P exists in mouse.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Kappa B Ras antibody has been tested for use in ELISA against the immunizing peptide.  The antibody is likely suitable for western blotting and other immunoassays. Specific conditions must be optimized by the investigator.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: KB-RAS

Background: Background: NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells.  It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. Upon stimulation, IkB is phosphorylated and degraded, and then NF-kB translocates into the nucleus to activate the transcription of target genes.  Two IkB proteins, IkBa and IkBb, exhibit divergent biochemical and genetic characteristics, exemplified by their different degradation kinetics.  These differences indicate their distinctive physiological functions. Mutated forms of the small G-protein Ras are found in about 30 % of all human cancers, including 95 % of pancreatic and 50 % of colon cancers.  KB-RAS is the most commonly mutated form of Ras.  KB-RAS was found to interact with the PEST domains of both IkB proteins in vitro.  NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.

Gene ID: 28512

UniProt: Q9NYS0