LPO antibody

Catalog No. ABIN103436

Supplier Product No.: 200-601-146-0100

The Sheep Polyclonal anti-LPO antibody has been validated for WB, IP and ELISA. It is suitable to detect LPO in samples from Cow.

Quick Overview for LPO antibody (ABIN103436)

Target: LPO (Lactoperoxidase (LPO))

Reactivity: Cow

Host: Sheep

Clonality: Polyclonal

Conjugate: This LPO antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-LPO Antibody

Supplier Product No.: 200-601-146-0100

Supplier: Rockland

Purpose: Lactoperoxidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk].

Characteristics: Synonyms: sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody

Purification: Anti-LACTOPEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Lactoperoxidase [Bovine Milk]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Lactoperoxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Lactoperoxidase [Bovine Milk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) code #613-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for LPO

Target: LPO (Lactoperoxidase (LPO))

Alternative Name: Lactoperoxidase

Background: Background: Lactoperoxidase is an antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN). It may protect the udder from infection and promote growth in newborn calves. It inhibits growth of the following bacterial species: E.coli, K.pneumoniae, P.aeruginosa, S.sonnei, S.saphrophyticus, S.epidermidis, and S.dysenteriae.

Gene ID: 280844

UniProt: P80025