LPO antibody (Biotin)

Catalog No. ABIN457588

This Rabbit Polyclonal antibody specifically detects LPO in WB, ICC, ELISA, DB and IHC (p). It exhibits reactivity toward Cow.

Quick Overview for LPO antibody (Biotin) (ABIN457588)

Target: LPO (Lactoperoxidase (LPO))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPO antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LPO Antibody (Biotin)

Purpose: Rabbit anti Bovine lactoperoxidase, conjugated with Biotin

Specificity: Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Bovine lactoperoxidase.

Cross-Reactivity (Details): The antiSerum does not cross-react with any other component of Bovine Serum. Interspecies cross-reactivity is a normal feature of antibodies, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, but reactivity with lactoperoxidase from Goat and Sheep may be expected.

Characteristics: The reactivity of the antiserum is restricted to lactoperoxidase as tested in immunoelectrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma. In immunocytochemical and immunohistochemical staining to identify and measure lactoperoxidase at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. As a second step an avidin or streptavidin conjugate of the user's choice has to be used.

Purification: Purified

Immunogen: Lactoperoxidase (EC 1.11.1.7) is one of the major enzymes in bovine milk. The enzyme has a molecular weight of 80,000. It sis isolated from pooled bovine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details for LPO

Target: LPO (Lactoperoxidase (LPO))

Alternative Name: Lactoperoxidase