VaChT Transporter (AA 521-532) antibody
The Mouse Monoclonal anti-VaChT Transporter antibody is suitable to detect VaChT Transporter in samples from Human. It has been validated for IHC, WB and IF/ICC.
Catalog No. ABIN1043018
$557.19
Plus shipping costs $50.00
100 μg
Shipping to:
United States
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Quick Overview for VaChT Transporter (AA 521-532) antibody (ABIN1043018)
Target
VaChT Transporter
Reactivity
Human
Host
Mouse
Clonality
Monoclonal
Conjugate
Un-conjugated
Application
Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)
Clone
S6-38
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Binding Specificity
- AA 521-532
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Purpose
- Anti-VAChT Transporter Mouse Monoclonal Antibody
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Specificity
- This antibody recognizes human, mouse, and rat VAChT.
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Cross-Reactivity
- Human, Mouse, Rat
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Purification
- Purified by Protein G affinity chromatography.
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Immunogen
- Synthetic peptide corresponding to aa 521-532 (CEDDYNYYYTRS) of human VAChT (accession no. NP_003046)
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Isotype
- IgG1
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Application Notes
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Immunoblotting: use at 1-10 μg/mL. A band of 55 kDa is detected. The molecular weight of glycosylated VAChT is 70 kDa.
Immunohistochemistry: use at 1-10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: rat brain -
Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Dilute in PBS or medium that is identical to that used in the assay system.
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Concentration
- 1.0 mg/mL
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Buffer
- PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
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- VaChT Transporter
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Background
- Vesicular acetylcholine transporter,Involved in acetylcholine transport into synaptic vesicles. {PubMed:8071310}.,VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.,Membrane, Multi-pass membrane protein,VAChT, Solute carrier family 18 member 3
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UniProt
- Q16572
Target
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