VaChT Transporter (AA 521-532) antibody
Reactivity: Human
IHC, WB, IF/ICC
Host: Mouse
Monoclonal
S6-38
unconjugated
-
- Target
- VaChT Transporter
- Binding Specificity
- AA 521-532
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
- Application
- Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)
- Purpose
- Anti-VAChT Transporter Mouse Monoclonal Antibody
- Specificity
- This antibody recognizes human, mouse, and rat VAChT.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified by Protein G affinity chromatography.
- Immunogen
- Synthetic peptide corresponding to aa 521-532 (CEDDYNYYYTRS) of human VAChT (accession no. NP_003046)
- Clone
- S6-38
- Isotype
- IgG1
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-
- Application Notes
-
Immunoblotting: use at 1-10 μg/mL. A band of 55 kDa is detected. The molecular weight of glycosylated VAChT is 70 kDa.
Immunohistochemistry: use at 1-10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: rat brain - Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
- Dilute in PBS or medium that is identical to that used in the assay system.
- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
-
- Target
- VaChT Transporter
- Background
- Vesicular acetylcholine transporter,Involved in acetylcholine transport into synaptic vesicles. {PubMed:8071310}.,VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.,Membrane, Multi-pass membrane protein,VAChT, Solute carrier family 18 member 3
- UniProt
- Q16572
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