VGlut2 Transporter (AA 501-582), (C-Term) antibody
Reactivity: Rat
IHC, WB, IF/ICC
Host: Mouse
Monoclonal
S29-29
unconjugated
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- Target
- VGlut2 Transporter
- Binding Specificity
- AA 501-582, C-Term
- Reactivity
- Rat
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)
- Purpose
- Anti-VGlut2 Transporter Mouse Monoclonal Antibody
- Specificity
- This antibody recognizes human, mouse, and rat VGlut2.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified by Protein G affinity chromatography.
- Immunogen
- Fusion protein corresponding to aa 501-582 (cytoplasmic C-terminus) of rat VGlut2 (accession no. NP_445879).
- Clone
- S29-29
- Isotype
- IgG1
-
- Application Notes
-
Immunoblotting: use at 1-2 μg/mL. A band of ~60 kDa is detected.
Immunohistochemistry: use at 1-5 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: rat brain lysate. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
- Dilute in PBS or medium that is identical to that used in the assay system.
- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
-
- Target
- VGlut2 Transporter
- Background
- Vesicular glutamate transporter 2,Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed:11551935, PubMed:11698619, PubMed:11698620, PubMed:11502256, PubMed:17046815, PubMed:18080752). May also mediate the transport of inorganic phosphate (PubMed:17046815). Involved in the regulation of retinal hyaloid vessel regression during postnatal development (By similarity). May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas (PubMed:11551935). {UniProtKB:Q8BLE7, PubMed:11502256, PubMed:11551935, PubMed:11698619, PubMed:11698620, PubMed:17046815, PubMed:18080752}.,The ATP-dependent, chloride-sensitive vesicular glutamate transporters include VGlut1, VGlut2, and VGlut3. VGlut1 and VGlut2 are expressed in brain in a complementary fashion. VGlut1 is expressed in the telencephalic regions whereas VGlut2 is expressed in the lower brainstem and diencephalic regions. VGlut3 is expressed in a small number of neurons in the striatum, hippocampus, cerebral cortex, and raphe nuclei. Pancreatic alpha and beta cells express VGlut1 and VGlut2 in response to glucose concentrations.,Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell junction, synapse, synaptosome,VGluT2, Differentiation-associated BNPI, Differentiation-associated Na(+-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6
- UniProt
- Q9JI12
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