gamma-Tubulin GCP2 (AA 2-194) antibody

Catalog No. ABIN1043030

The Mouse Monoclonal anti- antibody has been validated for ICC, IHC (p), IP and WB. It is suitable to detect in samples from Human, Mouse, Pig and Rat. There are 2+ publications available.

Quick Overview for gamma-Tubulin GCP2 (AA 2-194) antibody (ABIN1043030)

Target: gamma-Tubulin GCP2

Reactivity: Human, Mouse, Pig, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Clone: GCP2-01

Product Details

Binding Specificity: AA 2-194

Purpose: Anti-gamma-Tubulin GCP2 Purified

Specificity: The mouse monoclonal antibody GCP2-01 recognizes gamma-tubulin complex component 2 (GCP2), a 95-103 kDa intracellular protein. It recognizes also phosphorylated GCP2.

Cross-Reactivity (Details): Human, Porcine, Mouse, Rat

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: GST-fusion protein containing amino acids 2-194 of mouse GCP2

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Dráberová, DAgostino, Caracciolo, Sládková, Sulimenko, Sulimenko, Sobol, Maounis, Tzelepis, Mahera, K?en, Legido, Giordano, Mörk, Hozák, Dráber, Katsetos: "Overexpression and Nucleolar Localization of ?-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma." in: Journal of neuropathology and experimental neurology, Vol. 74, Issue 7, pp. 723-42, (2015) (PubMed).

Ho?ejší, Vinopal, Sládková, Dráberová, Sulimenko, Sulimenko, Vosecká, Philimonenko, Hozák, Katsetos, Dráber: "Nuclear ?-tubulin associates with nucleoli and interacts with tumor suppressor protein C53." in: Journal of cellular physiology, Vol. 227, Issue 1, pp. 367-82, (2011) (PubMed).

Target Details

Target: gamma-Tubulin GCP2

Background: Tubulin gamma complex associated protein 2,Gamma tubulin complex component 2 (GCP2) is 95-103 kDa protein which associates with two molecules of gamma tubulin and one molecule of gamma tubulin complex component 3 (GCP3), to form the gamma tubulin small complexes (gammaTuSCs). These complexes interact with GCP4, 5, and 6 to form the gamma tubulin ring complexes (gammaTuRCs), which are embedded in the microtubule organizing center matrix where they nucleate microtubules. The gammaTuRCs are also involved in the regulation of microtubule plus and minus end dynamics. Components of gamma tubulin complexes are highly conservative in eukaryotes.,GCP-2, GTCC2, GTC2

Gene ID: 10844

UniProt: Q9BSJ2