CDK2 antibody (C-Term)

Catalog No. ABIN1043673

Supplier Product No.: 100-401-161s

Product Details anti-CDK2 Antibody

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 100-401-161s

Supplier: Rockland

Purpose: cdk2 Antibody

Cross-Reactivity (Details): Antiserum will specifically react with a 33 kDa cdk2 protein from human, rat and mouse tissue.

Characteristics: Synonyms: rabbit anti-Cdk2 Antibody, Cdc2 related protein kinase antibody, Cdk 2 antibody, CDKN2 antibody, Cell division kinase 2 antibody, Cell division protein kinase 2 antibody, rabbit anti-Cyclin dependent kinase 2 antibody, p33 protein kinase antibody, cdk

Purification: Anti-cdk2 antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-cdk2 antibody was prepared from whole rabbit serum by repeated immunizations with a synthetic peptide corresponding to the C-terminus of the human cdk-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-cdk2 antibody has been tested in western blot and immunohistochemistry and is suitable for IF and other antibody based assays.  Multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Use paraffin embedded tissues for immunohistochemistry.  Optimal titers for other applications should be determined by the researcher.

Western Blot Dilution: 1:200 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:5,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2 (CDK2 Products)

Background: Background: Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Gene ID: 1017

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA