BIN3 antibody

Catalog No. ABIN1043741

Product Details anti-BIN3 Antibody

Target: BIN3 (Bridging Integrator 3 (BIN3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BIN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Anti-BIN3 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. BIN3 antibody is specific for human BIN3 protein. A BLAST analysis was used to suggest cross-reactivity with BIN3 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN3 from other sources has not been determined.

Characteristics: BIN-3 encodes an evolutionarily conserved and ubiquitously expressed member of the BAR superfamily of curved membrane and GTPase-binding proteins implicated in signal transduction and vesicular trafficking. In humans, Bin3 maps to chromosome 8p21.3, a region widely implicated in cancer suppression that is often deleted in non-Hodgkin’s lymphomas and various epithelial tumors. Study in mouse suggests that homozygous inactivation of Bin3 causes cataracts and an increased susceptibility to lymphomas during aging.

Sterility: Sterile filtered

Immunogen: Anti-BIN3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a GST tagged full legnth human BIN3 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein

Clone: 3A4

Isotype: IgG1

Application Details

Application Notes: Anti-BIN3 antibody has been tested for use in ELISA, Western Blot, and IP. Specific conditions for reactivity should be optimized by the end user.

Comment:

Gene Name: BIN3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References for anti-BIN3 antibody (ABIN1043741)

Routhier, Burn, Abbaszade, Summers, Albright, Prendergast: "Human BIN3 complements the F-actin localization defects caused by loss of Hob3p, the fission yeast homolog of Rvs161p." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21670-7, (2001) (PubMed).

Target Details for BIN3

Target: BIN3 (Bridging Integrator 3 (BIN3))

Alternative Name: BIN3 (BIN3 Products)

Synonyms: 1700015F07Rik antibody, bridging integrator 3 antibody, BIN3 antibody, Bin3 antibody

Background: BIN-3 encodes an evolutionarily conserved and ubiquitously expressed member of the BAR superfamily of curved membrane and GTPase-binding proteins implicated in signal transduction and vesicular trafficking. In humans, Bin3 maps to chromosome 8p21.3, a region widely implicated in cancer suppression that is often deleted in non–Hodgkin’s lymphomas and various epithelial tumors. Study in mouse suggests that homozygous inactivation of Bin3 causes cataracts and an increased susceptibility to lymphomas during aging.
Synonyms: Bridging integrator 3, BIN-3, BIN 3, BIN3 antibody, anti-BIN3 antibody

Gene ID: 55909

NCBI Accession: NP_061158

UniProt: Q9NQY0