AKT3 antibody (Internal Region)

Catalog No. ABIN1043749

Supplier Product No.: 200-301-e75

Product Details anti-AKT3 Antibody

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Supplier Product No.: 200-301-e75

Supplier: Rockland

Purpose: AKT3 Antibody

Cross-Reactivity (Details): Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein.

Characteristics: Synonyms: Mouse anti-AKT3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.

Immunogen Type: Conjugated Peptide

Clone: 25F6-F6-D8

Isotype: IgG1 kappa

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 20 μg/mL

Application Note: Anti-AKT3 Antibody is tested in ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Western Blot Dilution: 1:500-1:2000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-AKT3 antibody (ABIN1043749)

Bogen, Jensen, Hvalby, Walaas: "Glutamatergic neurotransmission in the synapsin I and II double knock-out mouse." in: Seminars in cell & developmental biology, Vol. 22, Issue 4, pp. 400-7, (2011) (PubMed).

Target Details for AKT3

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Alternative Name: AKT3 (AKT3 Products)

Background: Background: AKT3 Antibody detects AKT3 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT3 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

Gene ID: 10000

NCBI Accession: NP_001193658

UniProt: Q9Y243

Pathways: PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling