AKT3 antibody (Internal Region) (FITC)

Catalog No. ABIN5596898

Supplier Product No.: 200-302-e75

This Mouse Monoclonal antibody specifically detects AKT3 in WB, ELISA, IHC, DB, FACS and FM. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AKT3 antibody (Internal Region) (FITC) (ABIN5596898)

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT3 antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: 25F6-F6-D8

Product Details anti-AKT3 Antibody (FITC)

Binding Specificity: Internal Region

Supplier Product No.: 200-302-e75

Supplier: Rockland

Purpose: AKT3 FITC Antibody

Cross-Reactivity (Details): Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein.

Characteristics: Synonyms: Mouse anti-AKT3 antibody FITC conjugation, fluorescein conjugated Mouse anti-AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

Immunogen:

Immunogen: Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Labeling Ratio: 2.7

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-AKT3 FITC Antibody has been tested by ELISA and dot blot and is suitable for Flow Cytometry, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 50μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for AKT3

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Alternative Name: AKT3

Background: Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT3 (MOUSE) PE conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Gene ID: 10000

NCBI Accession: NP_001193658

UniProt: Q9Y243

Pathways: PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling