IL17F antibody (HRP)

Catalog No. ABIN1043827

Supplier Product No.: 210-403-b31

Product Details anti-IL17F Antibody (HRP)

Target: IL17F (Interleukin 17F (IL17F))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL17F antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 210-403-b31

Supplier: Rockland

Purpose: IL-17F Antibody Peroxidase Conjugated

Cross-Reactivity (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse IL17-F in cell supernatants and certain body fluids.

Characteristics: Synonyms: rabbit anti-IL-17F peroxidase conjugated antibody, rabbit anti-interleukin-17F peroxidase conjugated antibody, Interleukin17F, Interleukin-17F, ML1, ML-1, IL17F, IL24

Purification: This purified antibody has been heated to 56°C for 30 minutes.

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500-1:2,500

Application Note: This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 17.9 kDa in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:10,000-1:50,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IL17F antibody (HRP) (ABIN1043827)

Gomez-Rodriguez, Sahu, Handon, Davidson, Anderson, Kirby, August, Schwartzberg: "Differential expression of interleukin-17A and -17F is coupled to T cell receptor signaling via inducible T cell kinase." in: Immunity, Vol. 31, Issue 4, pp. 587-97, (2009) (PubMed).

Target Details for IL17F

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: Il17f (IL17F Products)

Background: Background: IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Gene ID: 257630

NCBI Accession: NP_665855

UniProt: Q7TNI7

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity