CCL20 antibody (Biotin)

Catalog No. ABIN1043839

Supplier Product No.: 210-506-b51

Product Details anti-CCL20 Antibody (Biotin)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CCL20 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Supplier Product No.: 210-506-b51

Supplier: Rockland

Purpose: Anti-Mouse MIP-3 alpha (RAT) Biotin Conjugated Antibody

Cross-Reactivity (Details): This antibody is specific for mouse MIP-3α protein.

Characteristics: Synonyms: rat anti-MIP-3α biotin conjugated antibody, CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

Purification: This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-MIP-3alpha (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse MIP-3alpha produced in E.coli followed by hybridoma development.

Immunogen Type: Recombinant Protein

Clone: 4N5F7

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Application Note: This purified antibody has been tested for use in ELISA, IP, western blot, and Neutralization. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 0.5 μg/mL

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CCL20 antibody (Biotin) (ABIN1043839)

Lisignoli, Manferdini, Codeluppi, Piacentini, Grassi, Cattini, Filardo, Facchini: "CCL20/CCR6 chemokine/receptor expression in bone tissue from osteoarthritis and rheumatoid arthritis patients: different response of osteoblasts in the two groups." in: Journal of cellular physiology, Vol. 221, Issue 1, pp. 154-60, (2009) (PubMed).

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: Ccl20 (CCL20 Products)

Background: Background: MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Gene ID: 20297

NCBI Accession: NP_001153210

UniProt: O89093

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection