Biotin antibody (FITC)

Catalog No. ABIN1043913

Supplier Product No.: 600-402-098

This anti-Biotin antibody is a Rabbit Polyclonal antibody detecting Biotin in WB and FM. Suitable for .

Quick Overview for Biotin antibody (FITC) (ABIN1043913)

Target: Biotin

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to FITC

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Product Details anti-Biotin Antibody (FITC)

Supplier Product No.: 600-402-098

Supplier: Rockland

Purpose: Biotin Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Biotinylated IgG and Biotinylated Bovine Serum Albumin.

Characteristics: Synonyms: rabbit anti-Biotin Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Biotin Antibody

Purification: Biotin Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotype: IgG

Labeling Ratio: 2.8

Application Details

Application Notes:

Application Note: Biotin Antibody Fluorescein Conjugated has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

IF Microscopy Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Fluorescein Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.